Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5276798..5277423 Replicon chromosome
Accession NZ_CP068618
Organism Klebsiella pneumoniae strain 41968

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JMZ79_RS25970 Protein ID WP_002882817.1
Coordinates 5276798..5277181 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JMZ79_RS25975 Protein ID WP_004150355.1
Coordinates 5277181..5277423 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMZ79_RS25955 5274164..5275066 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JMZ79_RS25960 5275063..5275698 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JMZ79_RS25965 5275695..5276624 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JMZ79_RS25970 5276798..5277181 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JMZ79_RS25975 5277181..5277423 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JMZ79_RS25980 5277628..5278545 + 918 WP_002882812.1 alpha/beta hydrolase -
JMZ79_RS25985 5278559..5279500 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JMZ79_RS25990 5279545..5279982 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JMZ79_RS25995 5279979..5280839 - 861 WP_002882807.1 virulence factor BrkB family protein -
JMZ79_RS26000 5280833..5281432 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T187445 WP_002882817.1 NZ_CP068618:c5277181-5276798 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T187445 NZ_CP089798:c4607614-4607303 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT187445 WP_004150355.1 NZ_CP068618:c5277423-5277181 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT187445 NZ_CP089798:c4607302-4607012 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTAAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGCTCACCGACAGGGCCAGCCAAAGCATTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References