Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4691645..4692455 Replicon chromosome
Accession NZ_CP068618
Organism Klebsiella pneumoniae strain 41968

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag JMZ79_RS23175 Protein ID WP_002887280.1
Coordinates 4691645..4692178 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JMZ79_RS23180 Protein ID WP_002887278.1
Coordinates 4692189..4692455 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMZ79_RS23170 4690476..4691597 + 1122 WP_002887282.1 cupin domain-containing protein -
JMZ79_RS23175 4691645..4692178 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
JMZ79_RS23180 4692189..4692455 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JMZ79_RS23185 4692558..4693991 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
JMZ79_RS23190 4693981..4694664 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
JMZ79_RS23195 4694836..4696221 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
JMZ79_RS23200 4696239..4696583 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T187442 WP_002887280.1 NZ_CP068618:c4692178-4691645 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T187442 NZ_CP089798:3619046-3619303 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAATGCCTCCGGCGCCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCACCAGTTACTCACATATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAACAGCTTTACAAGGCTGAACAGGTGGTTAAGGGGATG
CGGGACGTGATTGTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT187442 WP_002887278.1 NZ_CP068618:c4692455-4692189 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT187442 NZ_CP089798:c3619221-3618889 [Salmonella enterica subsp. enterica serovar Kentucky]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCTGCCA
GTCACCCTTAAGGTGCTGGCTGGGGATATGTGAGTAACTGGTGGTCATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGG
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTTAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGTACCGGGCGGTGATGAGCAACCGGCTACC
GGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References