Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5419595..5420220 Replicon chromosome
Accession NZ_CP068614
Organism Klebsiella pneumoniae strain 42362

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JMZ78_RS26840 Protein ID WP_002882817.1
Coordinates 5419595..5419978 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JMZ78_RS26845 Protein ID WP_004150355.1
Coordinates 5419978..5420220 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMZ78_RS26825 5416961..5417863 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JMZ78_RS26830 5417860..5418495 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JMZ78_RS26835 5418492..5419421 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JMZ78_RS26840 5419595..5419978 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JMZ78_RS26845 5419978..5420220 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JMZ78_RS26850 5420425..5421342 + 918 WP_002882812.1 alpha/beta hydrolase -
JMZ78_RS26855 5421356..5422297 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JMZ78_RS26860 5422342..5422779 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JMZ78_RS26865 5422776..5423636 - 861 WP_002882807.1 virulence factor BrkB family protein -
JMZ78_RS26870 5423630..5424229 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T187425 WP_002882817.1 NZ_CP068614:c5419978-5419595 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T187425 NZ_CP089797:864718-865131 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAAAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT187425 WP_004150355.1 NZ_CP068614:c5420220-5419978 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT187425 NZ_CP089797:864471-864737 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References