Detailed information of TA system TA187407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 20413..21056
• Replicon: plasmid p45706-R73.3
• Accession: NZ_CP068613
• Organism: Klebsiella pneumoniae strain 45706

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: JMZ75_RS26930
• Protein ID: WP_001044770.1
• Coordinates: 20413..20829 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: JMZ75_RS26935
• Protein ID: WP_001261282.1
• Coordinates: 20826..21056 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMZ75_RS26895	15906..16574	-	669	WP_001300294.1	EAL domain-containing protein	-
JMZ75_RS26900	16610..16846	-	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-
JMZ75_RS26905	16843..17205	-	363	WP_001277456.1	mercury resistance co-regulator MerD	-
JMZ75_RS26910	17223..18917	-	1695	WP_000105636.1	mercury(II) reductase	-
JMZ75_RS26915	18969..19391	-	423	WP_001340589.1	organomercurial transporter MerC	-
JMZ75_RS26920	19427..19537	-	111	Protein_22	mercuric transport protein periplasmic component	-
JMZ75_RS26925	19553..20250	-	698	Protein_23	IS1 family transposase	-
JMZ75_RS26930	20413..20829	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
JMZ75_RS26935	20826..21056	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JMZ75_RS26940	21013..21474	+	462	WP_072202616.1	hypothetical protein	-
JMZ75_RS26945	21618..21968	+	351	WP_000493378.1	hypothetical protein	-
JMZ75_RS26950	22019..22762	+	744	WP_000129823.1	hypothetical protein	-
JMZ75_RS26955	22759..23535	+	777	WP_000015958.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaOXA-9 / blaTEM-1A / aac(6')-Ib / ant(3'')-Ia / aadA2 / cmlA1 / aac(3)-IVa / aph(4)-Ia	-	1..73313	73313
	-	inside	IScluster/Tn	aac(6')-Ib / ant(3'')-Ia	-	19553..31856	12303

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T187407 WP_001044770.1 NZ_CP068613:c20829-20413 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T187407 NZ_CP089794:4151829-4152245 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAGTAAAACCTACATGCTGGATACCTGCATCTGTTCTTTCATTATGCGCGAACAGCCGGAAGCTGTGCTGAAACGCCT
TGAGCAGGCAGTGCTGCGCCGCCATCGTATTGTGGTGTCGGCGATTACCTACGCCGAAATGCGTTTTGGCTGTACGGGCA
AAAAGGCCTCGCCGCGCCATGCCCAACTGGTCGATGCATTCTGTAGTCGCCTGGATGCCGTGCTGGCCTGGGACCGGGCG
GCAGTGGATGCCACCACAGAGATCCGTGCCGTACTGGCCGCCGCCGGTACGCCGATTGGATCAAACGATGCTGCTATCGC
CGGCCATGCCATTGCGTCCGGGGCGATACTGGTCACTAATAACGTGAGGGAGTTTGAGCGGGTACCGGGTTTGCAGTACG
AAGATTGGGTAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT187407 WP_001261282.1 NZ_CP068613:c21056-20826 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT187407 NZ_CP089794:4151602-4151832 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCGAACCGTATCTGTATTTAAAAATGGCAATAACCGCGCGATCCGCCTTCCACGCGATCTGGATTTTGACGGCGTCAA
CGAACTTGAAATCTTCCGCGAAGGCGACACGATTATACTGCGCCCCGCCCGCCCTTCCTGGGGATCATTCCGTCATGAGG
AAAAAGCCAGCCCAGACTTTCTGACGGAACGCGAAGACATCATCAGCGATGAGGGACGCTTTGAACTATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3