Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4691568..4692378 Replicon chromosome
Accession NZ_CP068610
Organism Klebsiella pneumoniae strain 45706

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag JMZ75_RS23285 Protein ID WP_002887280.1
Coordinates 4691568..4692101 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JMZ75_RS23290 Protein ID WP_002887278.1
Coordinates 4692112..4692378 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMZ75_RS23280 4690399..4691520 + 1122 WP_002887282.1 cupin domain-containing protein -
JMZ75_RS23285 4691568..4692101 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
JMZ75_RS23290 4692112..4692378 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
JMZ75_RS23295 4692481..4693914 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
JMZ75_RS23300 4693904..4694587 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
JMZ75_RS23305 4694759..4696144 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
JMZ75_RS23310 4696162..4696506 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T187403 WP_002887280.1 NZ_CP068610:c4692101-4691568 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T187403 NZ_CP089794:2061002-2061105 [Salmonella enterica subsp. enterica serovar Kentucky]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT187403 WP_002887278.1 NZ_CP068610:c4692378-4692112 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT187403 NZ_CP089794:c2061107-2060964 [Salmonella enterica subsp. enterica serovar Kentucky]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References