Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 21068..21711 Replicon plasmid p59493-R72.9
Accession NZ_CP068609
Organism Klebsiella pneumoniae strain 59493

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag JMZ77_RS28380 Protein ID WP_001044770.1
Coordinates 21068..21484 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag JMZ77_RS28385 Protein ID WP_001261282.1
Coordinates 21481..21711 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMZ77_RS28345 16610..16846 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
JMZ77_RS28350 16843..17205 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
JMZ77_RS28355 17223..18917 - 1695 WP_000105636.1 mercury(II) reductase -
JMZ77_RS28360 18969..19391 - 423 WP_001340589.1 organomercurial transporter MerC -
JMZ77_RS28365 19427..19537 - 111 Protein_22 mercuric transport protein periplasmic component -
JMZ77_RS28370 19553..20250 - 698 Protein_23 IS1 family transposase -
JMZ77_RS28375 20284..20994 - 711 WP_004152334.1 ATP-binding protein -
JMZ77_RS28380 21068..21484 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
JMZ77_RS28385 21481..21711 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JMZ77_RS28390 21668..22129 + 462 WP_072202616.1 hypothetical protein -
JMZ77_RS28395 22273..22623 + 351 WP_000493378.1 hypothetical protein -
JMZ77_RS28400 22674..23417 + 744 WP_000129823.1 hypothetical protein -
JMZ77_RS28405 23414..24190 + 777 WP_000015958.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / blaTEM-1A / blaOXA-9 / aac(6')-Ib / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia - 1..72912 72912
- inside IScluster/Tn aac(6')-Ib - 19553..30029 10476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T187388 WP_001044770.1 NZ_CP068609:c21484-21068 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T187388 NZ_CP089792:299464-299982 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGGACAACCTGACAATAGAGATTCTGGCTGATAACGCAGAATACAACTGGCGGCAGTTCGACTGCGGTGAGGCATCATT
AAACCTTTTTTTAACGCAGCATCTTCAGCGCCAGCATAACAACAAAATCTTGCGCGGTTATGTACTCAGAACCACTACGC
CAGAACGGCGAGTGTTGGGGTATTACACCCTGTCAGGAAGCTGCTTTGAACGCGCGTCGTTGCCTTCCAGAACCCAGCAA
AAGCGCATCCCTTACCAGAATATCCCCAGCGTTACGCTGGGCCGTTTAGCGGTCGATCTCTCATTACAGGGAAAAGGGTG
GGGAGCTATATTGGTCACGCATGCGATGAAAGTGGTGTGGTCGGCATCACTGGCCGTTGGGATTCATGGCATCTTTGTGG
AAGCGTTAAACGAAAAAGCCCAGGCCTTTTATCAGCGGCTGGGCTTTATTTCTTTATCCGGAGAAAACGAGCACGCGCTG
TTTTACCCGACAAAGTCGATTGAGCAACTTTTTGGCTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT187388 WP_001261282.1 NZ_CP068609:c21711-21481 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT187388 NZ_CP089792:299191-299460 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGTCAGCCCTAAAAAAACAGCGTATCGATCTGCGGCTCACAGACGGCGATAAAAGCATGATTGAAGAAGCGGCCGCCAT
TACTAATCAATCAATCACGCAATTTATGTTGAATAGCGCAGCGGAGCGAGCCGCAGAAGTGCTTGAACAGCACCGTCGGG
TTATTCTTAATGAAGCATCCTGGGCGCGAGTCATGGATGCGCTTAGTCATCCTCCCACCCCCAATGAGAAATTAAAACGC
GCGGCGCAACGTCTACAAGACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References