Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 54864..55600 Replicon plasmid p59493-195.4
Accession NZ_CP068608
Organism Klebsiella pneumoniae strain 59493

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag JMZ77_RS27500 Protein ID WP_003026803.1
Coordinates 55118..55600 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JMZ77_RS27495 Protein ID WP_003026799.1
Coordinates 54864..55130 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMZ77_RS27450 50926..51288 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
JMZ77_RS27455 51338..51688 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
JMZ77_RS27460 52046..52315 + 270 WP_004152102.1 hypothetical protein -
JMZ77_RS27465 52303..52878 + 576 WP_004152103.1 hypothetical protein -
JMZ77_RS27470 52909..53403 + 495 WP_004152104.1 DNA-binding protein -
JMZ77_RS27475 53447..53815 + 369 WP_004152105.1 hypothetical protein -
JMZ77_RS27480 53849..54052 + 204 WP_004152106.1 hemolysin expression modulator Hha -
JMZ77_RS27485 54101..54358 + 258 WP_004152107.1 hypothetical protein -
JMZ77_RS27490 54434..54688 + 255 WP_004152108.1 hypothetical protein -
JMZ77_RS27495 54864..55130 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JMZ77_RS27500 55118..55600 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
JMZ77_RS27505 55812..57158 + 1347 WP_020314316.1 ISNCY family transposase -
JMZ77_RS27510 57659..58913 - 1255 Protein_58 IS3 family transposase -
JMZ77_RS27515 59001..59963 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1 - 1..195414 195414


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T187387 WP_003026803.1 NZ_CP068608:55118-55600 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T187387 NZ_CP089792:195437-195922 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGGGACATGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCAACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGAAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT187387 WP_003026799.1 NZ_CP068608:54864-55130 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT187387 NZ_CP089792:195162-195449 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References