Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 40787..41312 Replicon plasmid pEF7-17-58_3
Accession NZ_CP068594
Organism Escherichia coli strain EF7-18-58

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag JMY44_RS23815 Protein ID WP_001159871.1
Coordinates 40787..41092 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JMY44_RS23820 Protein ID WP_000813634.1
Coordinates 41094..41312 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMY44_RS23785 36409..36873 + 465 WP_001352013.1 helix-turn-helix domain-containing protein -
JMY44_RS23790 37049..37921 + 873 WP_000794337.1 hypothetical protein -
JMY44_RS23795 38079..38776 - 698 Protein_47 IS1 family transposase -
JMY44_RS23800 38833..39402 + 570 Protein_48 lipid II-degrading bacteriocin colicin M -
JMY44_RS23805 39452..39805 - 354 WP_000864813.1 colicin M immunity protein -
JMY44_RS23810 39977..40786 - 810 WP_000016963.1 site-specific integrase -
JMY44_RS23815 40787..41092 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
JMY44_RS23820 41094..41312 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JMY44_RS23825 41871..42797 + 927 WP_001290414.1 hypothetical protein -
JMY44_RS23830 42847..43101 + 255 WP_000678528.1 hypothetical protein -
JMY44_RS23835 43599..43943 + 345 WP_000792769.1 hypothetical protein -
JMY44_RS23840 44117..44410 - 294 Protein_56 hypothetical protein -
JMY44_RS23845 44464..45168 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
JMY44_RS23850 45230..45946 - 717 Protein_58 DUF4238 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid catA2 / blaCTX-M-55 / qnrS1 / aac(3)-IId - 1..82214 82214
- flank IS/Tn - - 44464..45168 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T187366 WP_001159871.1 NZ_CP068594:c41092-40787 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T187366 NZ_CP089790:c1032583-1032056 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCATGGATTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGACACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCGGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT187366 WP_000813634.1 NZ_CP068594:c41312-41094 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT187366 NZ_CP089790:c1032870-1032580 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References