Detailed information of TA system TA187363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 73614..74215
• Replicon: plasmid pEF7-18-58_1
• Accession: NZ_CP068592
• Organism: Escherichia coli strain EF7-18-58

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: JMY44_RS22990
• Protein ID: WP_001216034.1
• Coordinates: 73835..74215 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: JMY44_RS22985
• Protein ID: WP_001190712.1
• Coordinates: 73614..73835 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMY44_RS22965	72066..72377	+	312	WP_180397346.1	transposase	-
JMY44_RS22970	72374..72721	+	348	WP_072716716.1	IS66 family insertion sequence element accessory protein TnpB	-
JMY44_RS22975	72771..72977	+	207	Protein_64	IS66 family transposase	-
JMY44_RS22980	73152..73541	+	390	WP_000506730.1	DNA repair protein	-
JMY44_RS22985	73614..73835	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JMY44_RS22990	73835..74215	+	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
JMY44_RS22995	74220..74399	+	180	WP_038997082.1	hypothetical protein	-
JMY44_RS23000	74427..74891	+	465	Protein_69	hypothetical protein	-
JMY44_RS23005	74905..75602	-	698	Protein_70	IS1 family transposase	-
JMY44_RS23010	75658..76020	+	363	Protein_71	phage antirepressor KilAC domain-containing protein	-
JMY44_RS23015	76053..76904	-	852	WP_000611664.1	hypothetical protein	-
JMY44_RS23020	77015..77224	-	210	WP_000874156.1	hypothetical protein	-
JMY44_RS23025	77412..78392	+	981	WP_038989495.1	IS5-like element ISKpn26 family transposase	-
JMY44_RS23030	78507..79204	-	698	Protein_75	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	dfrA15	-	1..122138	122138
	-	inside	IScluster/Tn	-	-	68215..78392	10177

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T187363 WP_001216034.1 NZ_CP068592:73835-74215 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T187363 NZ_CP089790:195438-195923 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGGGACATGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCAACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGAAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT187363 WP_001190712.1 NZ_CP068592:73614-73835 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT187363 NZ_CP089790:195163-195450 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACATGTAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6