Detailed information of TA system TA187358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3557673..3558298
• Replicon: chromosome
• Accession: NZ_CP068591
• Organism: Escherichia coli strain EF7-18-58

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JMY44_RS16810
• Protein ID: WP_202763978.1
• Coordinates: 3557673..3558071 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: JMY44_RS16815
• Protein ID: WP_000450524.1
• Coordinates: 3558071..3558298 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMY44_RS16790	3553551..3553751	+	201	WP_000383836.1	YpfN family protein	-
JMY44_RS16795	3553861..3554559	-	699	WP_000679823.1	esterase	-
JMY44_RS16800	3554633..3556648	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
JMY44_RS16805	3556663..3557526	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
JMY44_RS16810	3557673..3558071	-	399	WP_202763978.1	tRNA(fMet)-specific endonuclease VapC	Toxin
JMY44_RS16815	3558071..3558298	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JMY44_RS16820	3558452..3559165	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
JMY44_RS16825	3559378..3560412	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
JMY44_RS16830	3560429..3561307	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JMY44_RS16835	3561453..3562025	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
JMY44_RS16840	3562025..3562495	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14924.28 Da
• Isoelectric Point: 9.2216

>T187358 WP_202763978.1 NZ_CP068591:c3558071-3557673 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHTRSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T187358 NZ_CP089789:3619169-3619426 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAATGCCTCCGGCGCCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCACCAGTTACTCACATATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAACAGCTTTACAAGGCTGAACAGGTGGTTAAGGGGATG
CGGGACGTGATTGTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT187358 WP_000450524.1 NZ_CP068591:c3558298-3558071 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT187358 NZ_CP089789:c3619344-3619012 [Salmonella enterica subsp. enterica serovar Kentucky]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCTGCCA
GTCACCCTTAAGGTGCTGGCTGGGGATATGTGAGTAACTGGTGGTCATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGG
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTTAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGTACCGGGCGGTGATGAGCAACCGGCTACC
GGGGCCGTATTTT

Structures

Antitoxin

• AlphaFold DB: A0A829CM33