Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1378717..1379554 Replicon chromosome
Accession NZ_CP068591
Organism Escherichia coli strain EF7-18-58

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag JMY44_RS06415 Protein ID WP_000227784.1
Coordinates 1378717..1379259 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag JMY44_RS06420 Protein ID WP_001297137.1
Coordinates 1379243..1379554 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMY44_RS06395 1374256..1375167 - 912 WP_000705847.1 2-dehydropantoate 2-reductase -
JMY44_RS06400 1375335..1375826 + 492 WP_001138904.1 nucleotide binding protein YajQ -
JMY44_RS06405 1375954..1377318 - 1365 WP_001000978.1 MFS transporter -
JMY44_RS06410 1377726..1378661 + 936 WP_001297127.1 sel1 repeat family protein -
JMY44_RS06415 1378717..1379259 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
JMY44_RS06420 1379243..1379554 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
JMY44_RS06425 1379739..1380629 - 891 WP_000971336.1 heme o synthase -
JMY44_RS06430 1380641..1380970 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JMY44_RS06435 1380970..1381584 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JMY44_RS06440 1381574..1383565 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JMY44_RS06445 1383587..1384534 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T187334 WP_000227784.1 NZ_CP068591:c1379259-1378717 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T187334 NZ_CP089781:3538901-3539245 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT187334 WP_001297137.1 NZ_CP068591:c1379554-1379243 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT187334 NZ_CP089781:3539242-3539571 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References