Detailed information of TA system TA187325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 913464..913984
• Replicon: chromosome
• Accession: NZ_CP068576
• Organism: Staphylococcus equorum strain KS1030

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: JL104_RS04275
• Protein ID: WP_040762585.1
• Coordinates: 913772..913984 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: J9GWP4
• Locus tag: JL104_RS04270
• Protein ID: WP_002507096.1
• Coordinates: 913464..913757 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL104_RS04245	908601..909392	+	792	WP_069819326.1	hydroxyethylthiazole kinase	-
JL104_RS04250	909392..910030	+	639	WP_002507092.1	thiamine phosphate synthase	-
JL104_RS04255	910118..910987	+	870	WP_002507093.1	membrane protein insertase YidC	-
JL104_RS04260	911058..911708	-	651	WP_202809225.1	HD domain-containing protein	-
JL104_RS04265	911836..913320	-	1485	WP_002507095.1	cardiolipin synthase	-
JL104_RS04270	913464..913757	+	294	WP_002507096.1	copper-sensing transcriptional repressor CsoR	Antitoxin
JL104_RS04275	913772..913984	+	213	WP_040762585.1	heavy-metal-associated domain-containing protein	Toxin
JL104_RS04280	914198..914338	+	141	WP_002507098.1	hypothetical protein	-
JL104_RS04285	914496..915698	-	1203	WP_002507099.1	rod shape-determining protein RodA	-
JL104_RS04290	915973..917043	+	1071	WP_002507100.1	D-alanine--D-alanine ligase	-
JL104_RS04295	917056..918411	+	1356	WP_002507101.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (18-67)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8130.16 Da
• Isoelectric Point: 4.5086

>T187325 WP_040762585.1 NZ_CP068576:913772-913984 [Staphylococcus equorum]
VITKVIYISGIENETQQKHIEQQLKQLHGVHQVNIEIESNNGKIFIDFETPASLNNLEKEVYDLGYEILY

Nucleotide

• Length: 213 bp

>T187325 NZ_CP089781:2917551-2917991 [Mycobacterium tuberculosis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11195.87 Da
• Isoelectric Point: 6.4791

>AT187325 WP_002507096.1 NZ_CP068576:913464-913757 [Staphylococcus equorum]
MAEIQKAHHSEQIKSNLKSRLNRIEGQVRAINRMVDEDVYCDDVLTQIRATRSALNGVATKLLDHHMKSCIMEKVEQGQQ
EEAMEELLVTFQKLMKD

Nucleotide

• Length: 294 bp

>AT187325 NZ_CP089781:2917277-2917564 [Mycobacterium tuberculosis]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Structures

Antitoxin

• AlphaFold DB: J9GWP4