Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 42920..43445 Replicon plasmid pKPC2-IR1251
Accession NZ_CP068575
Organism Klebsiella pneumoniae strain IR1251

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag JJV77_RS27795 Protein ID WP_013023785.1
Coordinates 43140..43445 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag JJV77_RS27790 Protein ID WP_001568025.1
Coordinates 42920..43138 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJV77_RS27760 38093..38790 - 698 Protein_44 IS1 family transposase -
JJV77_RS27765 39046..40068 - 1023 WP_000361404.1 helicase UvrD -
JJV77_RS27770 40053..41615 - 1563 WP_004206609.1 AAA family ATPase -
JJV77_RS27775 41689..42105 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
JJV77_RS27780 42102..42332 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JJV77_RS27785 42289..42750 + 462 WP_160866775.1 hypothetical protein -
JJV77_RS27790 42920..43138 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JJV77_RS27795 43140..43445 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
JJV77_RS27800 43614..44009 + 396 WP_017899885.1 hypothetical protein -
JJV77_RS27805 44036..44350 + 315 WP_053389906.1 hypothetical protein -
JJV77_RS27810 44361..45377 + 1017 WP_017899884.1 hypothetical protein -
JJV77_RS27815 45575..46369 + 795 WP_004197635.1 site-specific integrase -
JJV77_RS27820 46817..46996 - 180 WP_006788217.1 hypothetical protein -
JJV77_RS27825 47116..47742 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1C / blaKPC-2 - 1..86878 86878


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T187324 WP_013023785.1 NZ_CP068575:43140-43445 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T187324 NZ_CP089781:c2857330-2856935 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT187324 WP_001568025.1 NZ_CP068575:42920-43138 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT187324 NZ_CP089781:c2857572-2857327 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References