Detailed information of TA system TA187323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 41689..42332
• Replicon: plasmid pKPC2-IR1251
• Accession: NZ_CP068575
• Organism: Klebsiella pneumoniae strain IR1251

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: JJV77_RS27775
• Protein ID: WP_001044770.1
• Coordinates: 41689..42105 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: JJV77_RS27780
• Protein ID: WP_001261282.1
• Coordinates: 42102..42332 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJV77_RS27750	37015..37512	+	498	Protein_42	IS110-like element IS5075 family transposase	-
JJV77_RS27755	37792..38064	-	273	Protein_43	transposase	-
JJV77_RS27760	38093..38790	-	698	Protein_44	IS1 family transposase	-
JJV77_RS27765	39046..40068	-	1023	WP_000361404.1	helicase UvrD	-
JJV77_RS27770	40053..41615	-	1563	WP_004206609.1	AAA family ATPase	-
JJV77_RS27775	41689..42105	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
JJV77_RS27780	42102..42332	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JJV77_RS27785	42289..42750	+	462	WP_160866775.1	hypothetical protein	-
JJV77_RS27790	42920..43138	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
JJV77_RS27795	43140..43445	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	-
JJV77_RS27800	43614..44009	+	396	WP_017899885.1	hypothetical protein	-
JJV77_RS27805	44036..44350	+	315	WP_053389906.1	hypothetical protein	-
JJV77_RS27810	44361..45377	+	1017	WP_017899884.1	hypothetical protein	-
JJV77_RS27815	45575..46369	+	795	WP_004197635.1	site-specific integrase	-
JJV77_RS27820	46817..46996	-	180	WP_006788217.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1C / blaKPC-2	-	1..86878	86878

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T187323 WP_001044770.1 NZ_CP068575:c42105-41689 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T187323 NZ_CP089781:2856068-2856445 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT187323 WP_001261282.1 NZ_CP068575:c42332-42102 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT187323 NZ_CP089781:2855814-2856071 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3