Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 41689..42332 Replicon plasmid pKPC2-IR1251
Accession NZ_CP068575
Organism Klebsiella pneumoniae strain IR1251

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag JJV77_RS27775 Protein ID WP_001044770.1
Coordinates 41689..42105 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag JJV77_RS27780 Protein ID WP_001261282.1
Coordinates 42102..42332 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJV77_RS27750 37015..37512 + 498 Protein_42 IS110-like element IS5075 family transposase -
JJV77_RS27755 37792..38064 - 273 Protein_43 transposase -
JJV77_RS27760 38093..38790 - 698 Protein_44 IS1 family transposase -
JJV77_RS27765 39046..40068 - 1023 WP_000361404.1 helicase UvrD -
JJV77_RS27770 40053..41615 - 1563 WP_004206609.1 AAA family ATPase -
JJV77_RS27775 41689..42105 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
JJV77_RS27780 42102..42332 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JJV77_RS27785 42289..42750 + 462 WP_160866775.1 hypothetical protein -
JJV77_RS27790 42920..43138 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
JJV77_RS27795 43140..43445 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
JJV77_RS27800 43614..44009 + 396 WP_017899885.1 hypothetical protein -
JJV77_RS27805 44036..44350 + 315 WP_053389906.1 hypothetical protein -
JJV77_RS27810 44361..45377 + 1017 WP_017899884.1 hypothetical protein -
JJV77_RS27815 45575..46369 + 795 WP_004197635.1 site-specific integrase -
JJV77_RS27820 46817..46996 - 180 WP_006788217.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1C / blaKPC-2 - 1..86878 86878


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T187323 WP_001044770.1 NZ_CP068575:c42105-41689 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T187323 NZ_CP089781:2856068-2856445 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT187323 WP_001261282.1 NZ_CP068575:c42332-42102 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT187323 NZ_CP089781:2855814-2856071 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References