Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5218034..5218659 Replicon chromosome
Accession NZ_CP068572
Organism Klebsiella pneumoniae strain IR1251

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JJV77_RS25650 Protein ID WP_002882817.1
Coordinates 5218034..5218417 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JJV77_RS25655 Protein ID WP_004150355.1
Coordinates 5218417..5218659 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJV77_RS25635 5215400..5216302 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JJV77_RS25640 5216299..5216934 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JJV77_RS25645 5216931..5217860 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JJV77_RS25650 5218034..5218417 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JJV77_RS25655 5218417..5218659 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
JJV77_RS25660 5218864..5219781 + 918 WP_002882812.1 alpha/beta hydrolase -
JJV77_RS25665 5219795..5220736 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JJV77_RS25670 5220781..5221218 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
JJV77_RS25675 5221215..5222075 - 861 WP_002882807.1 virulence factor BrkB family protein -
JJV77_RS25680 5222069..5222668 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T187321 WP_002882817.1 NZ_CP068572:c5218417-5218034 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T187321 NZ_CP089781:c2841894-2841475 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT187321 WP_004150355.1 NZ_CP068572:c5218659-5218417 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT187321 NZ_CP089781:c2842115-2841891 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References