Detailed information of TA system TA187302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1207906..1208823
• Replicon: chromosome
• Accession: NZ_CP068563
• Organism: Bacillus velezensis strain GUMT319

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: JKJ03_RS06345
• Protein ID: WP_057079950.1
• Coordinates: 1208077..1208823 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: JKJ03_RS06340
• Protein ID: WP_003154807.1
• Coordinates: 1207906..1208076 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKJ03_RS06305	1203139..1204761	+	1623	WP_166706517.1	pyocin knob domain-containing protein	-
JKJ03_RS06310	1204774..1205145	+	372	WP_070081681.1	hypothetical protein	-
JKJ03_RS06315	1205150..1205347	+	198	WP_168237133.1	XkdX family protein	-
JKJ03_RS06320	1205404..1206165	+	762	WP_070081683.1	phage portal protein	-
JKJ03_RS06325	1206217..1206480	+	264	WP_003154815.1	hemolysin XhlA family protein	-
JKJ03_RS06330	1206494..1206757	+	264	WP_003154813.1	phage holin	-
JKJ03_RS06335	1206771..1207649	+	879	WP_045207177.1	N-acetylmuramoyl-L-alanine amidase	-
JKJ03_RS06340	1207906..1208076	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
JKJ03_RS06345	1208077..1208823	-	747	WP_057079950.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
JKJ03_RS06350	1208928..1209926	-	999	WP_003154805.1	inorganic phosphate transporter	-
JKJ03_RS06355	1209939..1210556	-	618	WP_003154804.1	DUF47 domain-containing protein	-
JKJ03_RS06360	1210842..1212158	-	1317	WP_003154801.1	amino acid permease	-
JKJ03_RS06365	1212481..1213431	+	951	WP_039251754.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29033.52 Da
• Isoelectric Point: 4.7755

>T187302 WP_057079950.1 NZ_CP068563:c1208823-1208077 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T187302 NZ_CP089781:1381804-1382235 [Mycobacterium tuberculosis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT187302 WP_003154807.1 NZ_CP068563:c1208076-1207906 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT187302 NZ_CP089781:1381547-1381807 [Mycobacterium tuberculosis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14