Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 478158..478795 Replicon chromosome
Accession NZ_CP068563
Organism Bacillus velezensis strain GUMT319

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag JKJ03_RS02440 Protein ID WP_003156187.1
Coordinates 478445..478795 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag JKJ03_RS02435 Protein ID WP_003156188.1
Coordinates 478158..478439 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKJ03_RS02415 474523..475122 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
JKJ03_RS02420 475215..475580 + 366 WP_071392104.1 holo-ACP synthase -
JKJ03_RS02425 475745..476752 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
JKJ03_RS02430 476869..478038 + 1170 WP_202735847.1 alanine racemase -
JKJ03_RS02435 478158..478439 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
JKJ03_RS02440 478445..478795 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JKJ03_RS02445 478913..479734 + 822 WP_003156182.1 STAS domain-containing protein -
JKJ03_RS02450 479739..480104 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
JKJ03_RS02455 480107..480508 + 402 WP_003156178.1 anti-sigma regulatory factor -
JKJ03_RS02460 480520..481527 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
JKJ03_RS02465 481590..481919 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
JKJ03_RS02470 481916..482398 + 483 WP_003156173.1 anti-sigma B factor RsbW -
JKJ03_RS02475 482364..483152 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
JKJ03_RS02480 483152..483754 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T187301 WP_003156187.1 NZ_CP068563:478445-478795 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T187301 NZ_CP089781:1236939-1237313 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT187301 WP_003156188.1 NZ_CP068563:478158-478439 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT187301 NZ_CP089781:1236745-1236942 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References