Detailed information of TA system TA187253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 80955..81598
• Replicon: plasmid pB
• Accession: NZ_CP068446
• Organism: Klebsiella quasipneumoniae strain SWMUF35

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: IT777_RS26985
• Protein ID: WP_130983600.1
• Coordinates: 81182..81598 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: IT777_RS26980
• Protein ID: WP_130983601.1
• Coordinates: 80955..81185 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IT777_RS26945	75964..76161	+	198	WP_044296379.1	hypothetical protein	-
IT777_RS26950	76518..77384	-	867	WP_004118283.1	replication initiation protein	-
IT777_RS26955	77919..78023	+	105	WP_032409716.1	hypothetical protein	-
IT777_RS26960	78152..78409	+	258	WP_000764642.1	hypothetical protein	-
IT777_RS26965	78467..79243	-	777	WP_000015958.1	site-specific integrase	-
IT777_RS26970	79240..79983	-	744	WP_004098973.1	hypothetical protein	-
IT777_RS26975	80034..80384	-	351	WP_000493378.1	hypothetical protein	-
IT777_RS26980	80955..81185	+	231	WP_130983601.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IT777_RS26985	81182..81598	+	417	WP_130983600.1	type II toxin-antitoxin system VapC family toxin	Toxin
IT777_RS26990	81644..85447	-	3804	WP_188028209.1	pcar	-
IT777_RS26995	85795..86043	+	249	WP_032415726.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..90038	90038

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15052.55 Da
• Isoelectric Point: 9.2957

>T187253 WP_130983600.1 NZ_CP068446:81182-81598 [Klebsiella quasipneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVTLRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T187253 NZ_CP089780:c2191801-2191505 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8751.77 Da
• Isoelectric Point: 4.3533

>AT187253 WP_130983601.1 NZ_CP068446:80955-81185 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMTEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT187253 NZ_CP089780:c2192049-2191798 [Mycobacterium tuberculosis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGGTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA