Detailed information of TA system TA187250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2593011..2594599
• Replicon: chromosome
• Accession: NZ_CP068444
• Organism: Klebsiella quasipneumoniae strain SWMUF35

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: IT777_RS12635
• Protein ID: WP_135733131.1
• Coordinates: 2593011..2594333 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2N4W0X3
• Locus tag: IT777_RS12640
• Protein ID: WP_023289667.1
• Coordinates: 2594333..2594599 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IT777_RS12620	2588056..2590568	+	2513	Protein_2466	fimbrial biogenesis outer membrane usher protein	-
IT777_RS12625	2590556..2591560	+	1005	WP_135733133.1	type 1 fimbrial protein	-
IT777_RS12630	2591702..2592994	+	1293	WP_135733132.1	glycoside hydrolase family 10 protein	-
IT777_RS12635	2593011..2594333	-	1323	WP_135733131.1	type II toxin-antitoxin system HipA family toxin	Toxin
IT777_RS12640	2594333..2594599	-	267	WP_023289667.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
IT777_RS12645	2594984..2595310	-	327	WP_064154632.1	hydroxyisourate hydrolase	-
IT777_RS12650	2595307..2595807	-	501	WP_032452825.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
IT777_RS12655	2595785..2597164	-	1380	WP_065812518.1	purine permease	-
IT777_RS12660	2597510..2598664	+	1155	WP_023289663.1	FAD-dependent urate hydroxylase HpxO	-
IT777_RS12665	2598645..2599568	-	924	WP_044522995.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49213.51 Da
• Isoelectric Point: 8.6110

>T187250 WP_135733131.1 NZ_CP068444:c2594333-2593011 [Klebsiella quasipneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDDEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLATLRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATRGRKTEINAIYPRHFLATAKAVNFPRDQMLAILDEFADRVP
QAIESARRTLPADFSAHVWRAITENMLKLHARLQQGLQAR

Nucleotide

• Length: 1323 bp

>T187250 NZ_CP089780:1963754-1964002 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9891.25 Da
• Isoelectric Point: 7.3163

>AT187250 WP_023289667.1 NZ_CP068444:c2594599-2594333 [Klebsiella quasipneumoniae]
MHSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETATAP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT187250 NZ_CP089780:1963542-1963754 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N4W0X3