Detailed information of TA system TA187234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 118251..118786
• Replicon: plasmid unnamed2
• Accession: NZ_CP068442
• Organism: Pantoea agglomerans strain ZJU23

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A8X8DPA1
• Locus tag: JK642_RS20555
• Protein ID: WP_010257115.1
• Coordinates: 118251..118538 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JK642_RS20560
• Protein ID: WP_039387444.1
• Coordinates: 118535..118786 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK642_RS20520 (JK642_20470)	113609..114304	-	696	WP_083070625.1	haloacid dehalogenase type II	-
JK642_RS20525 (JK642_20475)	114586..114753	-	168	Protein_97	type II toxin-antitoxin system RelE/ParE family toxin	-
JK642_RS20530 (JK642_20480)	114950..115870	+	921	WP_069027204.1	Rpn family recombination-promoting nuclease/putative transposase	-
JK642_RS20535 (JK642_20485)	115967..116251	-	285	Protein_99	IS3 family transposase	-
JK642_RS20540 (JK642_20490)	116271..116501	-	231	Protein_100	helix-turn-helix domain-containing protein	-
JK642_RS20545 (JK642_20495)	116758..117576	+	819	WP_235765257.1	DUF4225 domain-containing protein	-
JK642_RS20550 (JK642_20500)	117573..117887	-	315	WP_143790132.1	hypothetical protein	-
JK642_RS20555 (JK642_20505)	118251..118538	-	288	WP_010257115.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JK642_RS20560 (JK642_20510)	118535..118786	-	252	WP_039387444.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JK642_RS20565 (JK642_20515)	119077..120210	-	1134	WP_235765258.1	glutathione-independent formaldehyde dehydrogenase	-
JK642_RS20570 (JK642_20520)	120503..121453	-	951	WP_182688119.1	sulfonate ABC transporter substrate-binding protein	-
JK642_RS20575 (JK642_20525)	121549..123438	-	1890	WP_235765259.1	sigma-54-dependent Fis family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..170989	170989

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11173.08 Da
• Isoelectric Point: 10.3856

>T187234 WP_010257115.1 NZ_CP068442:c118538-118251 [Pantoea agglomerans]
VSYTVKFREEAFKEWQKLDKSLQQQFAKKLKKCCDNPHIPSARLRGIKDCYKIKLRASGFRLVYQVIDDQLVIAVVAVGK
RERSDVYHLASERMR

Nucleotide

• Length: 288 bp

>T187234 NZ_CP089780:716714-717121 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9108.38 Da
• Isoelectric Point: 3.9507

>AT187234 WP_039387444.1 NZ_CP068442:c118786-118535 [Pantoea agglomerans]
MPNIILSDTSASVSELKKNPMATVNAGDGYPVAILNRNQPAFYCVPAELYEQMLDALDDQELVKLVSERSGQRLLDVDLD
ALL

Nucleotide

• Length: 252 bp

>AT187234 NZ_CP089780:716457-716717 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA