Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 979948..980565 Replicon chromosome
Accession NZ_CP068440
Organism Pantoea agglomerans strain ZJU23

Toxin (Protein)


Gene name Hha Uniprot ID E0LTU7
Locus tag JK642_RS04415 Protein ID WP_003850458.1
Coordinates 979948..980163 (-) Length 72 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID E0LTU8
Locus tag JK642_RS04420 Protein ID WP_003850455.1
Coordinates 980188..980565 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JK642_RS04385 (JK642_04375) 975870..976208 + 339 WP_003850469.1 P-II family nitrogen regulator -
JK642_RS04390 (JK642_04380) 976243..977529 + 1287 WP_010255877.1 ammonium transporter AmtB -
JK642_RS04395 (JK642_04385) 977579..978442 - 864 WP_010255880.1 acyl-CoA thioesterase II -
JK642_RS04400 (JK642_04390) 978633..979190 + 558 WP_010255881.1 YbaY family lipoprotein -
JK642_RS04405 (JK642_04395) 979230..979541 - 312 WP_235764833.1 MGMT family protein -
JK642_RS04415 (JK642_04405) 979948..980163 - 216 WP_003850458.1 HHA domain-containing protein Toxin
JK642_RS04420 (JK642_04410) 980188..980565 - 378 WP_003850455.1 Hha toxicity modulator TomB Antitoxin
JK642_RS04425 (JK642_04415) 980713..981066 - 354 WP_031592173.1 hypothetical protein -
JK642_RS04430 (JK642_04420) 981394..982062 + 669 WP_069026576.1 ABC transporter ATP-binding protein -
JK642_RS04435 (JK642_04425) 982059..982898 + 840 WP_235764834.1 metal ABC transporter permease -
JK642_RS04440 (JK642_04430) 982914..983792 + 879 WP_031592168.1 metal ABC transporter substrate-binding protein -
JK642_RS04445 (JK642_04435) 983838..983978 - 141 WP_010255896.1 type B 50S ribosomal protein L36 -
JK642_RS04450 (JK642_04440) 983990..984244 - 255 WP_010671219.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(12-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8510.90 Da        Isoelectric Point: 9.4825

>T187230 WP_003850458.1 NZ_CP068440:c980163-979948 [Pantoea agglomerans]
MNKTLTKTDYLMRLRRCRSLDTLERVIEKNKYELPEDELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Download         Length: 216 bp

>T187230 NZ_CP089780:c695999-695586 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 14636.25 Da        Isoelectric Point: 4.3976

>AT187230 WP_003850455.1 NZ_CP068440:c980565-980188 [Pantoea agglomerans]
MDEYSPKRHDIAQLKYLCESLFDDSMATLTDSHHGWVNDPTSESNLQLNDLIEHIASFTMNYKIKHVEDEALISQIDEYL
DDTFMLFSNYGVNSPDLQRWQRSAKRLFNLFTEECAFLQQPSHSL

Download         Length: 378 bp

>AT187230 NZ_CP089780:c696232-695996 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I5AGC3

Antitoxin

Source ID Structure
AlphaFold DB A0A1I5AG55

References