Detailed information of TA system TA187219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 10..9384
• Replicon: plasmid pSJF2-e
• Accession: NZ_CP068424
• Organism: Gluconobacter sphaericus strain SJF2-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IGS75_RS15275
• Protein ID: WP_202259161.1
• Coordinates: 10..333 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: IGS75_RS15270
• Protein ID: WP_202259160.1
• Coordinates: 9384..13 (+)
• Length: -3123.3333333333 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGS75_RS15275	10..333	+	324	WP_202259161.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IGS75_RS15280	341..1285	+	945	WP_202259172.1	tyrosine-type recombinase/integrase	-
IGS75_RS15285	1288..1818	-	531	WP_202259174.1	hypothetical protein	-
IGS75_RS15290	2109..2594	-	486	WP_202259176.1	helix-turn-helix domain-containing protein	-
IGS75_RS15295	3045..3476	+	432	Protein_5	hypothetical protein	-
IGS75_RS15300	3609..4028	+	420	WP_202259178.1	toxin-antitoxin system HicB family antitoxin	-
IGS75_RS15305	4262..4993	+	732	WP_202259186.1	plasmid recombination protein	-
IGS75_RS15310	5084..5416	-	333	WP_202259154.1	helix-turn-helix domain-containing protein	-
IGS75_RS15315	6306..6773	-	468	WP_202259156.1	hypothetical protein	-
IGS75_RS15320	7298..7892	+	595	Protein_10	IS5 family transposase	-
IGS75_RS15325	8293..8820	+	528	WP_202259158.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..9589	9589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11420.30 Da
• Isoelectric Point: 10.2947

>T187219 WP_202259161.1 NZ_CP068424:10-333 [Gluconobacter sphaericus]
MNRGDFVTVALQGDLGKPRPALIVQADRFETTASVTILPVTSTLIDAPLLRLSVAPDERNGLRKTSQVMIDKPSTVSRDK
IGPTFGIASDTVMLSVNRALALFLGLA

Nucleotide

• Length: 324 bp

>T187219 NZ_CP089779:c3892466-3892164 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: -3123.3333333333 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT187219 WP_202259160.1 NZ_CP068424:9384-13 [Gluconobacter sphaericus]

Nucleotide

• Length: -9370 bp

>AT187219 NZ_CP089779:c3892116-3891895 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA