Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 9408..9946 Replicon plasmid pSJF2-a
Accession NZ_CP068420
Organism Gluconobacter sphaericus strain SJF2-1

Toxin (Protein)


Gene name mazF Uniprot ID A0A5C0A8C0
Locus tag IGS75_RS13470 Protein ID WP_062022288.1
Coordinates 9623..9946 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1B6VPH7
Locus tag IGS75_RS13465 Protein ID WP_062269019.1
Coordinates 9408..9626 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGS75_RS13440 5330..5965 + 636 WP_202258726.1 hypothetical protein -
IGS75_RS13445 5962..6303 + 342 WP_202258728.1 hypothetical protein -
IGS75_RS13450 6300..6608 + 309 WP_202258729.1 hypothetical protein -
IGS75_RS13455 6798..7223 + 426 WP_202258731.1 hypothetical protein -
IGS75_RS13460 7277..9139 + 1863 WP_202258733.1 ParB/RepB/Spo0J family partition protein -
IGS75_RS13465 9408..9626 + 219 WP_062269019.1 antitoxin MazE family protein Antitoxin
IGS75_RS13470 9623..9946 + 324 WP_062022288.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IGS75_RS13475 10427..11568 + 1142 WP_202256546.1 IS3 family transposase -
IGS75_RS13480 11659..12327 + 669 WP_194258308.1 hypothetical protein -
IGS75_RS13485 12396..13010 + 615 WP_202258735.1 hypothetical protein -
IGS75_RS13490 13030..13455 + 426 WP_202258737.1 hypothetical protein -
IGS75_RS13495 13711..13887 + 177 WP_202258739.1 hypothetical protein -
IGS75_RS13500 13965..14474 + 510 WP_202258741.1 DUF3085 domain-containing protein -
IGS75_RS13505 14455..14703 + 249 WP_202258743.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..162291 162291


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-106)

Antitoxin

Antitoxin MazE-like

(7-70)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11436.30 Da        Isoelectric Point: 10.2947

>T187218 WP_062022288.1 NZ_CP068420:9623-9946 [Gluconobacter sphaericus]
MNRGDFVTVALQGDLGKPRPALIVQADRFETTASVTVLPVTSTLIDAPLLRLSVTPDERNGLRKTSQVMIDKPSTVSRDK
IGPTFGIASDTVMLSVNRALALFLGLA

Download         Length: 324 bp

>T187218 NZ_CP089779:c3891898-3891473 [Mycobacterium tuberculosis]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8104.19 Da        Isoelectric Point: 6.9785

>AT187218 WP_062269019.1 NZ_CP068420:9408-9626 [Gluconobacter sphaericus]
MTPVAIRVQKRRDTLRKAGLRPVQIWVPDTRTPGFDAECQRQARLVAEAERHDPALMSFVESAASDLDGWDA

Download         Length: 219 bp

>AT187218 NZ_CP089779:c3892116-3891895 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5C0A8C0

Antitoxin

Source ID Structure
AlphaFold DB A0A1B6VPH7

References