Detailed information of TA system TA187194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1579008..1580596
• Replicon: chromosome
• Accession: NZ_CP068394
• Organism: Escherichia coli strain M-17

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A192ENQ5
• Locus tag: JK368_RS07675
• Protein ID: WP_001125432.1
• Coordinates: 1579008..1580330 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V0VAH2
• Locus tag: JK368_RS07680
• Protein ID: WP_001304368.1
• Coordinates: 1580330..1580596 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK368_RS07645	1574210..1575274	-	1065	WP_001519742.1	alpha/beta fold hydrolase	-
JK368_RS07650	1575432..1576193	-	762	WP_001066620.1	carboxymuconolactone decarboxylase family protein	-
JK368_RS07655	1576171..1576649	-	479	Protein_1500	cupin domain-containing protein	-
JK368_RS07660	1577038..1577385	-	348	WP_001337832.1	hypothetical protein	-
JK368_RS07665	1577387..1577881	-	495	WP_001337831.1	AEC family transporter	-
JK368_RS07670	1578328..1578852	+	525	Protein_1503	acetylxylan esterase	-
JK368_RS07675	1579008..1580330	-	1323	WP_001125432.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JK368_RS07680	1580330..1580596	-	267	WP_001304368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JK368_RS07685	1580904..1581662	+	759	WP_001286543.1	trans-aconitate 2-methyltransferase	-
JK368_RS07690	1581666..1582580	-	915	WP_001313828.1	bestrophin family inner membrane protein	-
JK368_RS07695	1582777..1584228	-	1452	WP_000854639.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49297.02 Da
• Isoelectric Point: 8.6972

>T187194 WP_001125432.1 NZ_CP068394:c1580330-1579008 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T187194 NZ_CP089779:c2491642-2491238 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9957.31 Da
• Isoelectric Point: 4.9632

>AT187194 WP_001304368.1 NZ_CP068394:c1580596-1580330 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Nucleotide

• Length: 267 bp

>AT187194 NZ_CP089779:c2491851-2491648 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• AlphaFold DB: A0A192ENQ5

Antitoxin

• AlphaFold DB: V0VAH2