Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 517764..518382 Replicon chromosome
Accession NZ_CP068394
Organism Escherichia coli strain M-17

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JK368_RS02445 Protein ID WP_001291435.1
Coordinates 517764..517982 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JK368_RS02450 Protein ID WP_000344800.1
Coordinates 518008..518382 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JK368_RS02410 513051..513623 + 573 WP_000779831.1 YbaY family lipoprotein -
JK368_RS02415 513654..513965 - 312 WP_000409908.1 MGMT family protein -
JK368_RS02425 514344..514697 + 354 WP_000878140.1 DUF1428 domain-containing protein -
JK368_RS02430 514739..516289 - 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JK368_RS02435 516453..516923 - 471 WP_000136192.1 YlaC family protein -
JK368_RS02440 517039..517590 - 552 WP_000102539.1 maltose O-acetyltransferase -
JK368_RS02445 517764..517982 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JK368_RS02450 518008..518382 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JK368_RS02455 518927..522076 - 3150 WP_046960538.1 efflux RND transporter permease AcrB -
JK368_RS02460 522099..523292 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T187192 WP_001291435.1 NZ_CP068394:c517982-517764 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T187192 NZ_CP089779:c2287228-2286980 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT187192 WP_000344800.1 NZ_CP068394:c518382-518008 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT187192 NZ_CP089779:c2287440-2287228 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References