Detailed information of TA system TA187184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2335203..2335788
• Replicon: chromosome
• Accession: NZ_CP068390
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain YS21

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: JHW28_RS10745
• Protein ID: WP_202339686.1
• Coordinates: 2335495..2335788 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A3G7ICX6
• Locus tag: JHW28_RS10740
• Protein ID: WP_038580367.1
• Coordinates: 2335203..2335493 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JHW28_RS10725	2330556..2333036	+	2481	WP_202340112.1	FtsX-like permease family protein	-
JHW28_RS10730	2333026..2334102	+	1077	WP_202339685.1	iron ABC transporter permease	-
JHW28_RS10735	2334216..2334803	+	588	WP_096373676.1	hypothetical protein	-
JHW28_RS10740	2335203..2335493	-	291	WP_038580367.1	putative addiction module antidote protein	Antitoxin
JHW28_RS10745	2335495..2335788	-	294	WP_202339686.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JHW28_RS10750	2336023..2337417	-	1395	WP_202339687.1	VOC family protein	-
JHW28_RS10755	2337682..2338806	+	1125	WP_202339688.1	diguanylate cyclase	-
JHW28_RS10760	2338900..2340144	+	1245	WP_038580359.1	M20/M25/M40 family metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-96)

Antitoxin

• Uncharacterized protein HI1420 (8-94)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10686.19 Da
• Isoelectric Point: 10.3456

>T187184 WP_202339686.1 NZ_CP068390:c2335788-2335495 [Pseudomonas chlororaphis subsp. aurantiaca]
MDYEILQTTVFAQWHTTQRDLRARIAIARRIERASAGNLGDVKNFGGGLSKMRVNTGAGYHLYFTGRGNTLIVLLLGGDK
SSQPADICQARSLAKEF

Nucleotide

• Length: 294 bp

>T187184 NZ_CP089779:c1935515-1935105 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10239.78 Da
• Isoelectric Point: 8.0096

>AT187184 WP_038580367.1 NZ_CP068390:c2335493-2335203 [Pseudomonas chlororaphis subsp. aurantiaca]
MSDTLVPFDIAALLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKTTGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVSRG

Nucleotide

• Length: 291 bp

>AT187184 NZ_CP089779:c1935741-1935508 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G7ICX6