Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1870962..1871598 Replicon chromosome
Accession NZ_CP068389
Organism Bacillus altitudinis strain ws31

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag JKL50_RS09890 Protein ID WP_003214169.1
Coordinates 1870962..1871312 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag JKL50_RS09895 Protein ID WP_003214273.1
Coordinates 1871317..1871598 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKL50_RS09850 1865995..1866594 - 600 WP_008346897.1 SpoIIE family protein phosphatase -
JKL50_RS09855 1866594..1867382 - 789 WP_025206796.1 RNA polymerase sigma factor SigB -
JKL50_RS09860 1867348..1867836 - 489 WP_007496471.1 anti-sigma B factor RsbW -
JKL50_RS09865 1867833..1868162 - 330 WP_008346902.1 anti-sigma factor antagonist -
JKL50_RS09870 1868222..1869229 - 1008 WP_202734694.1 PP2C family protein-serine/threonine phosphatase -
JKL50_RS09875 1869240..1869641 - 402 WP_003214085.1 anti-sigma regulatory factor -
JKL50_RS09880 1869644..1870012 - 369 WP_003214235.1 RsbT antagonist protein RsbS -
JKL50_RS09885 1870017..1870847 - 831 WP_007496484.1 STAS domain-containing protein -
JKL50_RS09890 1870962..1871312 - 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JKL50_RS09895 1871317..1871598 - 282 WP_003214273.1 hypothetical protein Antitoxin
JKL50_RS09900 1871892..1873073 - 1182 WP_202733937.1 alanine racemase -
JKL50_RS09905 1873202..1874218 - 1017 WP_007496489.1 outer membrane lipoprotein-sorting protein -
JKL50_RS09910 1874379..1874744 - 366 WP_202733938.1 holo-ACP synthase -
JKL50_RS09915 1874839..1875444 + 606 WP_007496491.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T187180 WP_003214169.1 NZ_CP068389:c1871312-1870962 [Bacillus altitudinis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T187180 NZ_CP068389:c1871312-1870962 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAAATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT187180 WP_003214273.1 NZ_CP068389:c1871598-1871317 [Bacillus altitudinis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT187180 NZ_CP068389:c1871598-1871317 [Bacillus altitudinis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References