Detailed information of TA system TA187177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 2705..3227
• Replicon: plasmid unnamed1
• Accession: NZ_CP068387
• Organism: Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum strain R51

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: JI728_RS23085
• Protein ID: WP_000220560.1
• Coordinates: 2705..2986 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: JI728_RS23090
• Protein ID: WP_000121743.1
• Coordinates: 2976..3227 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI728_RS23050 (JI728_22900)	594..866	-	273	WP_269724649.1	hypothetical protein	-
JI728_RS23055 (JI728_22905)	879..1028	-	150	WP_269724650.1	hypothetical protein	-
JI728_RS23060 (JI728_22910)	1098..1277	-	180	WP_000439078.1	hypothetical protein	-
JI728_RS23065 (JI728_22915)	1320..1649	-	330	WP_000866648.1	hypothetical protein	-
JI728_RS23070 (JI728_22920)	1742..1957	-	216	WP_001180116.1	hypothetical protein	-
JI728_RS23075	2051..2191	-	141	WP_269724652.1	hypothetical protein	-
JI728_RS23080 (JI728_22930)	2236..2559	-	324	WP_001181903.1	hypothetical protein	-
JI728_RS23085 (JI728_22935)	2705..2986	-	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JI728_RS23090 (JI728_22940)	2976..3227	-	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
JI728_RS23095 (JI728_22945)	4462..5301	+	840	WP_001352874.1	replication initiation protein	-
JI728_RS23100 (JI728_22950)	5322..6164	+	843	WP_000435064.1	replication initiation protein	-
JI728_RS23105 (JI728_22955)	6168..6614	+	447	WP_001074384.1	hypothetical protein	-
JI728_RS23110 (JI728_22960)	6751..7107	+	357	WP_000876433.1	DNA distortion polypeptide 3	-
JI728_RS23115	7159..7755	-	597	WP_000135520.1	YcxB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / blaTEM-1B	-	1..67734	67734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T187177 WP_000220560.1 NZ_CP068387:c2986-2705 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T187177 NZ_CP089779:1392710-1393105 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT187177 WP_000121743.1 NZ_CP068387:c3227-2976 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT187177 NZ_CP089779:1392468-1392713 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301