Detailed information of TA system TA187160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 992025..992575
• Replicon: chromosome
• Accession: NZ_CP068386
• Organism: Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum strain R51

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A764IVN5
• Locus tag: JI728_RS04760
• Protein ID: WP_001199742.1
• Coordinates: 992267..992575 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7RP97
• Locus tag: JI728_RS04755
• Protein ID: WP_001118105.1
• Coordinates: 992025..992264 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI728_RS04720 (987464)	987464..988495	-	1032	WP_000453347.1	L-idonate 5-dehydrogenase	-
JI728_RS04725 (988712)	988712..989242	+	531	WP_000896758.1	gluconokinase	-
JI728_RS04730 (989270)	989270..990289	-	1020	WP_000152558.1	NAD(P)-dependent alcohol dehydrogenase	-
JI728_RS04740 (991046)	991046..991477	+	432	Protein_922	helix-turn-helix domain-containing protein	-
JI728_RS04745 (991508)	991508..991591	+	84	WP_228958541.1	DUF4942 domain-containing protein	-
JI728_RS04750 (991668)	991668..991916	+	249	WP_000168388.1	ribbon-helix-helix domain-containing protein	-
JI728_RS04755 (992025)	992025..992264	+	240	WP_001118105.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JI728_RS04760 (992267)	992267..992575	+	309	WP_001199742.1	CcdB family protein	Toxin
JI728_RS04765 (992981)	992981..993121	+	141	Protein_927	Arm DNA-binding domain-containing protein	-
JI728_RS04770 (993153)	993153..994286	-	1134	Protein_928	IS3 family transposase	-
JI728_RS04775 (994607)	994607..995137	+	531	WP_000909461.1	SEF14 fimbria major subunit SefA	-
JI728_RS04780 (995259)	995259..995999	+	741	WP_001575372.1	SEF14/SEF18 fimbria chaperone SefB	-
JI728_RS04785 (996016)	996016..997050	+	1035	WP_223610907.1	fimbria/pilus outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	991091..994194	3103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11826.62 Da
• Isoelectric Point: 6.4785

>T187160 WP_001199742.1 NZ_CP068386:992267-992575 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
MQYMVYHNKGNSKAYPYLLDVQSDIIDELHTRMVIPLFPVSRLVNNPVKRLTPTLNVEGNDYLVMTHEMASIRLSQIGDE
VMDVRSHRQTIKNALDFIFDGF

Nucleotide

• Length: 309 bp

>T187160 NZ_CP089778:3783130-3783387 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8719.90 Da
• Isoelectric Point: 6.4633

>AT187160 WP_001118105.1 NZ_CP068386:992025-992264 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
MPGMSVRHKKTVSVTLEPALLQQARDAGINLSAVLTAALKEEISATGVERWKAENRAGLQELNRITDEHGLLSDDYRTF

Nucleotide

• Length: 240 bp

>AT187160 NZ_CP089778:3782858-3783133 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A764IVN5

Antitoxin

• AlphaFold DB: A0A5H9SZK8