Detailed information of TA system TA187154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 305886..306472
• Replicon: chromosome
• Accession: NZ_CP068386
• Organism: Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum strain R51

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A3V9X0E4
• Locus tag: JI728_RS01405
• Protein ID: WP_000174964.1
• Coordinates: 306104..306472 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: V7IU48
• Locus tag: JI728_RS01400
• Protein ID: WP_001522145.1
• Coordinates: 305886..306107 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI728_RS01375 (300907)	300907..302016	+	1110	WP_000822976.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
JI728_RS01380 (302076)	302076..303002	+	927	WP_079786686.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
JI728_RS01385 (302999)	302999..304276	+	1278	WP_000803764.1	branched chain amino acid ABC transporter permease LivM	-
JI728_RS01390 (304273)	304273..305040	+	768	WP_000082080.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
JI728_RS01395 (305042)	305042..305755	+	714	WP_000416114.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
JI728_RS01400 (305886)	305886..306107	+	222	WP_001522145.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JI728_RS01405 (306104)	306104..306472	+	369	WP_000174964.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
JI728_RS01410 (306731)	306731..308047	+	1317	WP_000624755.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
JI728_RS01415 (308152)	308152..309039	+	888	WP_000099303.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
JI728_RS01420 (309036)	309036..309881	+	846	WP_000572194.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
JI728_RS01425 (309883)	309883..310953	+	1071	WP_000907835.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	300907..311690	10783

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-114)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.91 Da
• Isoelectric Point: 6.7252

>T187154 WP_000174964.1 NZ_CP068386:306104-306472 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
MTLQLISAEEIIQFHDRLLRVTPGVTGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLAANPDFVDMTVDAAAGRLTLEQIVVRLRA

Nucleotide

• Length: 369 bp

>T187154 NZ_CP089778:c3143881-3142997 [Mycobacterium tuberculosis]
GTGGCCGGTCTGACCCGTGCGCTCGTTGCGCGTCACGCTCTTGGTCGGGCCGAGGCATACGACGCCGCTCTGCTCGACGT
CGCCCAGGACCACCTGCTGTACCTGCTGTCGCAGACCGTCCAGTTCGGCGATAACCGCCTGGTGTTCAAAGGCGGTACCA
GCTTACGGAAGTGCCGGCTGGGCAACGTCGGGCGGTTCTCCACTGATCTGGACTTCAGCGCGCCCGACGATGAGGTCGTG
CTGGAGGTGTGCGAGCTGATCGACGGCGCCCGTGTCGGCGGCTTCGAATTCGGCGTGCAGAGCACACGAGGCGACGGCCG
GCATTGGCAGTTGCGGGTGCGGCACACGGAATTGGGCGAGCCCCGGATCGTCGCCAGCGTCGAGTTCGCACGGCGGCCGT
TGGCTTTGCCGTCTGAGCTGCTCGCATTCATCCAGTTGCCGATCCATAAGGCTTACGGTTTTGGGCTGCCGACACTGCCG
GTCGTGGCAGAAGCGGAAGCCTGCGCAGAGAAACTCGCGCGGTACCGCCGCGTTGCGCTGGCACGGGACCTGTACGACCT
GAACCACTTCGCCTCGCGAACGATTGACGAACCGCTCGTGCGGCGGCTGTGGGTGCTCAAGGTGTGGGGTGATGTCGTCG
ATGACCGGCGCGGCACCCGGCCACTACGCGTCGAAGACGTCCTCGCCGCCCGCAGCGAGCACGACTTCCAGCCCGACTCG
ATCGGCGTGCTGACCCGTCCTGTCGCTATGGCTGCCTGGGAAGCTCGCGTTCGGAAGCGATTTGCGTTCCTCACTGACCT
CGACGCCGACGAGCAGCGGTGGGCCGCCTGCGACGAACGGCACCGCCGCGAAGTGGAGAACGCGCTGGCGGTGCTGCGGT
CCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8166.17 Da
• Isoelectric Point: 5.0775

>AT187154 WP_001522145.1 NZ_CP068386:305886-306107 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
MRTVNYSEARQNLAEVLESAVTAGPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGNELRELADK

Nucleotide

• Length: 222 bp

>AT187154 NZ_CP089778:c3144771-3143884 [Mycobacterium tuberculosis]
GTGGTGAGCCCAGCCGGCGCCGATCGGCGCATCCCCACGTGGGCGTCACGGGTGGTGTCCGGCCTCGCTCGCGATCGGCC
CGTAGTCGTCACGAAAGAGGATCTGACGCAACGGCTTACAGAGGCTGGGTGTGGACGCGACCCCGACTCCGCCATCCGCG
AACTGCGCCGCATTGGGTGGCTGGTCCAACTGCCGGTGAAAGGCACCTGGGCATTCATTCCCCCCGGCGAGGCCGCCATC
TCGGACCCGTATCTACCGCTGCGCTCGTGGCTGGCCCGTGACCAGAACGCGGGCTTCATGCTGGCCGGTGCATCCGCAGC
GTGGCACCTCGGATACCTGGACCGCCAACCCGACGGCCGCATCCCGATCTGGCTGCCGCCGGCCAAAAGGCTGCCCGACG
GCCTAGCATCGTATGTGTCCGTCGTGCGCATCCCCTGGAACGCGGCGGACACCGCACTGCTGGCTCCGCGTCCCGCGTTG
CTGGTCCGGCGGCGGCTCGACCTCGTCGCGTGGGCGACCGGGTTACCGGCGCTAGGACCAGAAGCATTACTTGTGCAAAT
CGCCACGCGCCCGGCCTCGTTCGGGCCGTGGGCCGACCTTGTCCCCCACCTTGACGACCTCGTCGCCGACTGCAGTGACG
AGCGCCTGGAGCGTCTACTGTCGGGCCGGCCGACATCGGCCTGGCAGCGTGCAAGCTACCTGCTGGACTCGGGCGGTGAA
CCGGCACGAGGCCAGGCGCTGCTGGCCAAGCGGCACACCGAAGTGATGCCGGTGACTCGATTCACCACCGCGCACAGCCG
CGACCGTGGCGAGAGTGTCTGGGCTCCCGAGTATCAGCTTGTCGACGAGCTCGTCGTACCGCTGCTGCGCGTGATCGGCA
AGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A3V9X0E4