Detailed information of TA system TA187147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 163910..164436
• Replicon: plasmid p1575-1
• Accession: NZ_CP068288
• Organism: Enterobacter hormaechei strain 1575

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: JJL14_RS23385
• Protein ID: WP_000323025.1
• Coordinates: 163910..164197 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: JJL14_RS23390
• Protein ID: WP_000534858.1
• Coordinates: 164197..164436 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJL14_RS23350	159480..159656	-	177	WP_001371930.1	hypothetical protein	-
JJL14_RS23355	160167..161111	+	945	WP_000778029.1	DUF5417 domain-containing protein	-
JJL14_RS23360	161207..161809	+	603	WP_012695474.1	hypothetical protein	-
JJL14_RS23365	161869..162219	+	351	WP_000743059.1	hypothetical protein	-
JJL14_RS23370	162266..162469	+	204	WP_001015183.1	hypothetical protein	-
JJL14_RS23375	162751..163071	+	321	WP_000332796.1	hypothetical protein	-
JJL14_RS23380	163680..163838	-	159	WP_001447866.1	type I toxin-antitoxin system Hok family toxin	-
JJL14_RS23385	163910..164197	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
JJL14_RS23390	164197..164436	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
JJL14_RS23395	164699..165622	-	924	WP_000167917.1	cation diffusion facilitator family transporter	-
JJL14_RS23400	165822..166394	-	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
JJL14_RS23405	166870..168108	-	1239	WP_000219087.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-1 / qnrS1 / dfrA14 / aac(3)-IId / blaTEM-1B / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 / aph(3')-Ia / tet(D) / blaSFO-1 / mph(A)	htpB	1..342447	342447

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T187147 WP_000323025.1 NZ_CP068288:c164197-163910 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T187147 NZ_CP089778:2879324-2879737 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT187147 WP_000534858.1 NZ_CP068288:c164436-164197 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT187147 NZ_CP089778:2879776-2880033 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8