Detailed information of TA system TA187134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 1427526..1428136
• Replicon: plasmid pRscUGMSS_Db01
• Accession: NZ_CP068286
• Organism: Ralstonia syzygii subsp. celebesensis strain UGMSS_Db01

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JK151_RS22850
• Protein ID: WP_078223783.1
• Coordinates: 1427828..1428136 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JK151_RS22845
• Protein ID: WP_013209163.1
• Coordinates: 1427526..1427831 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK151_RS22815	1422917..1423201	-	285	WP_078223782.1	hypothetical protein	-
JK151_RS22820	1423534..1423721	+	188	Protein_1295	transposase	-
JK151_RS22825	1423888..1424557	+	670	Protein_1296	hypothetical protein	-
JK151_RS22830	1424662..1425165	-	504	Protein_1297	MFS transporter	-
JK151_RS22835	1425259..1426469	+	1211	WP_099091583.1	IS3 family transposase	-
JK151_RS22840	1426500..1427315	-	816	Protein_1299	MFS transporter	-
JK151_RS22845	1427526..1427831	-	306	WP_013209163.1	putative addiction module antidote protein	Antitoxin
JK151_RS22850	1427828..1428136	-	309	WP_078223783.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JK151_RS22855	1428221..1429420	-	1200	WP_013209165.1	MFS transporter	-
JK151_RS22860	1429548..1430363	-	816	WP_013209166.1	GntR family transcriptional regulator	-
JK151_RS22865	1430537..1431088	-	552	WP_078223784.1	helix-turn-helix transcriptional regulator	-
JK151_RS22870	1431220..1431814	+	595	Protein_1305	GNAT family N-acetyltransferase	-
JK151_RS22875	1432071..1432505	+	435	WP_013209169.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cheB / cheW / motA / katA / acrB / cheY	1..1614911	1614911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11821.56 Da
• Isoelectric Point: 9.0017

>T187134 WP_078223783.1 NZ_CP068286:c1428136-1427828 [Ralstonia syzygii subsp. celebesensis]
MLTIRTTEIFDDWFYSLRDKAAQRRVQVRIDRLQMGNPGDVKTVREGIREMRIDHGPGYRVYVAQRGVVLIVLLCGGDKS
TQEADVRRAIDLSRRLDVDNME

Nucleotide

• Length: 309 bp

>T187134 NZ_CP089778:1968440-1968688 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11009.67 Da
• Isoelectric Point: 6.7174

>AT187134 WP_013209163.1 NZ_CP068286:c1427831-1427526 [Ralstonia syzygii subsp. celebesensis]
MMMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARARGMTQIARETGMTREGLYKALSEDGNP
SFATVLKVIRALGIRLHAVPA

Nucleotide

• Length: 306 bp

>AT187134 NZ_CP089778:1968228-1968440 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA