Detailed information of TA system TA187133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 3444505..3446024
• Replicon: chromosome
• Accession: NZ_CP068285
• Organism: Ralstonia syzygii subsp. celebesensis strain UGMSS_Db01

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: JK151_RS15640
• Protein ID: WP_078222737.1
• Coordinates: 3445101..3446024 (+)
• Length: 308 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: JK151_RS15635
• Protein ID: WP_078222738.1
• Coordinates: 3444505..3445104 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK151_RS15630	3440335..3444405	-	4071	WP_078222739.1	helix-turn-helix domain-containing protein	-
JK151_RS15635	3444505..3445104	+	600	WP_078222738.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
JK151_RS15640	3445101..3446024	+	924	WP_078222737.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JK151_RS15645	3446271..3447481	+	1211	WP_099091583.1	IS3 family transposase	-
JK151_RS15650	3447733..3449004	-	1272	WP_172840182.1	IS256 family transposase	-
JK151_RS15655	3449586..3450857	+	1272	WP_172840182.1	IS256 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3438757..3440079	1322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (39-275)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (6-50)

Sequences

Toxin

Protein

• Length: 308 a.a.
• Molecular weight: 34682.77 Da
• Isoelectric Point: 5.9899

>T187133 WP_078222737.1 NZ_CP068285:3445101-3446024 [Ralstonia syzygii subsp. celebesensis]
MNTTAPKHSATSIRARLFNHAKERGDDFQRVLTRYAIERLLFRLSQTEAVERYVLKGAMLFVTWPEHVFRPSGDLDLLGQ
GNPDPDAITELFTRICQVDVADDGIVFEPATLKVEPVREADKYQGVRLSLKGELAKAVIHVQVDIGFGDYVYPPPKYHIF
PSLLQDLPAANILMYPPETVVAEKFEAMIRFGEANGRIKDFHDMWVMTRTFSFDMPTLVEAVGGTLRRRETAIPTEMPAG
LTETFAKVAEERGLWSGFLRRNPPTLQPPPFADLQEELRRFFGPVITNLALPEGAKGRWDPDAGAWR

Nucleotide

• Length: 924 bp

>T187133 NZ_CP089778:c1947886-1947497 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22406.09 Da
• Isoelectric Point: 9.9958

>AT187133 WP_078222738.1 NZ_CP068285:3444505-3445104 [Ralstonia syzygii subsp. celebesensis]
MTYRERALSLIRDRGIARARDFKAAAIPLVYLKRLTDAGDLVRLGRGLYQDPERVGEDIAHDLAEAARLVPNGVISLISA
LRHHQLTTQLPHAVWITIPHKARTPNLQGLRLEIIRATDEVLTAGVEHIRVEGVDVPIYCVAKTIADCFKHRRHVGEDVA
IEALRDALRQKKTTVSELMKYAAIDRVAKRIEPYIKAMQ

Nucleotide

• Length: 600 bp

>AT187133 NZ_CP089778:c1948110-1947883 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA