Detailed information of TA system TA187126

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 25347..25972
• Replicon: plasmid pCY598
• Accession: NZ_CP068284
• Organism: Escherichia coli strain CY598

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JI738_RS22990
• Protein ID: WP_000911324.1
• Coordinates: 25574..25972 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: JI738_RS22985
• Protein ID: WP_000450532.1
• Coordinates: 25347..25574 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI738_RS22985 (25347)	25347..25574	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JI738_RS22990 (25574)	25574..25972	+	399	WP_000911324.1	type II toxin-antitoxin system VapC family toxin	Toxin
JI738_RS22995 (25981)	25981..28134	-	2154	WP_000009350.1	type IV conjugative transfer system coupling protein TraD	-
JI738_RS23000 (28387)	28387..29118	-	732	WP_000850422.1	conjugal transfer complement resistance protein TraT	-
JI738_RS23005 (29143)	29143..29664	-	522	WP_000632670.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..67408	67408

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14800.09 Da
• Isoelectric Point: 7.8604

>T187126 WP_000911324.1 NZ_CP068284:25574-25972 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMEVIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T187126 NZ_CP089778:1239640-1240014 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT187126 WP_000450532.1 NZ_CP068284:25347-25574 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT187126 NZ_CP089778:1239446-1239643 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5