Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3090271..3090925 Replicon chromosome
Accession NZ_CP068283
Organism Escherichia coli strain CY598

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JI738_RS15215 Protein ID WP_000244777.1
Coordinates 3090271..3090678 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JI738_RS15220 Protein ID WP_000354046.1
Coordinates 3090659..3090925 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI738_RS15195 (3086228) 3086228..3087961 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
JI738_RS15200 (3087967) 3087967..3088677 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JI738_RS15205 (3088702) 3088702..3089598 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JI738_RS15210 (3089710) 3089710..3090231 + 522 WP_001055874.1 flavodoxin FldB -
JI738_RS15215 (3090271) 3090271..3090678 - 408 WP_000244777.1 protein YgfX Toxin
JI738_RS15220 (3090659) 3090659..3090925 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JI738_RS15225 (3091168) 3091168..3092148 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JI738_RS15230 (3092344) 3092344..3093003 - 660 WP_000250274.1 hemolysin III family protein -
JI738_RS15235 (3093167) 3093167..3093478 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JI738_RS15240 (3093523) 3093523..3094956 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
JI738_RS15245 (3095013) 3095013..3095756 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T187110 WP_000244777.1 NZ_CP068283:c3090678-3090271 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T187110 NZ_CP089778:c641770-641357 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT187110 WP_000354046.1 NZ_CP068283:c3090925-3090659 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT187110 NZ_CP089778:c642033-641767 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References