Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2144832..2145663 Replicon chromosome
Accession NZ_CP068283
Organism Escherichia coli strain CY598

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag JI738_RS10780 Protein ID WP_000854814.1
Coordinates 2145289..2145663 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag JI738_RS10775 Protein ID WP_001285584.1
Coordinates 2144832..2145200 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI738_RS10760 (2142499) 2142499..2144031 + 1533 WP_001350525.1 protein YeeR -
JI738_RS10765 (2144028) 2144028..2144474 + 447 WP_000187523.1 RadC family protein -
JI738_RS10770 (2144537) 2144537..2144758 + 222 WP_000692323.1 DUF987 domain-containing protein -
JI738_RS10775 (2144832) 2144832..2145200 + 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
JI738_RS10780 (2145289) 2145289..2145663 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
JI738_RS10785 (2145660) 2145660..2145854 + 195 WP_000988600.1 DUF5983 family protein -
JI738_RS10790 (2145900) 2145900..2145980 + 81 Protein_2117 hypothetical protein -
JI738_RS10795 (2146269) 2146269..2146397 - 129 Protein_2118 transposase domain-containing protein -
JI738_RS10800 (2146517) 2146517..2146651 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
JI738_RS10805 (2146752) 2146752..2147081 - 330 WP_000450409.1 DUF496 family protein -
JI738_RS10810 (2147253) 2147253..2148311 - 1059 WP_001200891.1 FUSC family protein -
JI738_RS10815 (2148509) 2148509..2148982 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
JI738_RS10820 (2149101) 2149101..2150267 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T187105 WP_000854814.1 NZ_CP068283:2145289-2145663 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T187105 NZ_CP089778:71807-72208 [Mycobacterium tuberculosis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT187105 WP_001285584.1 NZ_CP068283:2144832-2145200 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT187105 NZ_CP089778:71575-71814 [Mycobacterium tuberculosis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References