Detailed information of TA system TA187067

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3333829..3334660
• Replicon: chromosome
• Accession: NZ_CP068281
• Organism: Escherichia coli strain CY706

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: JJW04_RS16390
• Protein ID: WP_000854814.1
• Coordinates: 3334286..3334660 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: JJW04_RS16385
• Protein ID: WP_001285584.1
• Coordinates: 3333829..3334197 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJW04_RS16370 (3331496)	3331496..3333028	+	1533	WP_001350525.1	protein YeeR	-
JJW04_RS16375 (3333025)	3333025..3333471	+	447	WP_000187523.1	RadC family protein	-
JJW04_RS16380 (3333534)	3333534..3333755	+	222	WP_000692323.1	DUF987 domain-containing protein	-
JJW04_RS16385 (3333829)	3333829..3334197	+	369	WP_001285584.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
JJW04_RS16390 (3334286)	3334286..3334660	+	375	WP_000854814.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
JJW04_RS16395 (3334657)	3334657..3334851	+	195	WP_000988600.1	DUF5983 family protein	-
JJW04_RS16400 (3334897)	3334897..3334977	+	81	Protein_3204	hypothetical protein	-
JJW04_RS16405 (3335266)	3335266..3335394	-	129	Protein_3205	transposase domain-containing protein	-
JJW04_RS16410 (3335514)	3335514..3335648	+	135	WP_001297944.1	EutP/PduV family microcompartment system protein	-
JJW04_RS16415 (3335749)	3335749..3336078	-	330	WP_000450409.1	DUF496 family protein	-
JJW04_RS16420 (3336250)	3336250..3337308	-	1059	WP_001200891.1	FUSC family protein	-
JJW04_RS16425 (3337506)	3337506..3337979	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
JJW04_RS16430 (3338098)	3338098..3339264	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T187067 WP_000854814.1 NZ_CP068281:3334286-3334660 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T187067 NZ_CP089777:c1235916-1235605 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACACCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT187067 WP_001285584.1 NZ_CP068281:3333829-3334197 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT187067 NZ_CP089777:c1236236-1235916 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A1M2E8G6