Detailed information of TA system TA187056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2752587..2753225
• Replicon: chromosome
• Accession: NZ_CP068281
• Organism: Escherichia coli strain CY706

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JJW04_RS13415
• Protein ID: WP_000813794.1
• Coordinates: 2752587..2752763 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JJW04_RS13420
• Protein ID: WP_001270286.1
• Coordinates: 2752809..2753225 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJW04_RS13395 (2748206)	2748206..2749420	-	1215	WP_001326689.1	BenE family transporter YdcO	-
JJW04_RS13400 (2749473)	2749473..2750009	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
JJW04_RS13405 (2750082)	2750082..2752043	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JJW04_RS13410 (2752135)	2752135..2752365	-	231	WP_000494244.1	YncJ family protein	-
JJW04_RS13415 (2752587)	2752587..2752763	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JJW04_RS13420 (2752809)	2752809..2753225	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JJW04_RS13425 (2753304)	2753304..2754710	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
JJW04_RS13430 (2754955)	2754955..2756100	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
JJW04_RS13435 (2756118)	2756118..2757131	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
JJW04_RS13440 (2757132)	2757132..2758073	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T187056 WP_000813794.1 NZ_CP068281:2752587-2752763 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T187056 NZ_CP089777:708109-708510 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT187056 WP_001270286.1 NZ_CP068281:2752809-2753225 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT187056 NZ_CP089777:707867-708112 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• PDB: 6HPB