Detailed information of TA system TA187044

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 1471037..1471716
• Replicon: chromosome
• Accession: NZ_CP068281
• Organism: Escherichia coli strain CY706

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: JJW04_RS06995
• Protein ID: WP_000854672.1
• Coordinates: 1471037..1471378 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: JJW04_RS07000
• Protein ID: WP_000070395.1
• Coordinates: 1471399..1471716 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJW04_RS06970 (1466314)	1466314..1466715	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
JJW04_RS06975 (1466754)	1466754..1467809	-	1056	WP_000749863.1	phosphoporin PhoE	-
JJW04_RS06980 (1468097)	1468097..1469200	+	1104	WP_001285288.1	glutamate 5-kinase	-
JJW04_RS06985 (1469212)	1469212..1470465	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
JJW04_RS06995 (1471037)	1471037..1471378	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
JJW04_RS07000 (1471399)	1471399..1471716	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
JJW04_RS07005 (1471735)	1471735..1471956	-	222	WP_000691994.1	DUF987 domain-containing protein	-
JJW04_RS07010 (1471965)	1471965..1472441	-	477	WP_000811693.1	RadC family protein	-
JJW04_RS07015 (1472457)	1472457..1472915	-	459	WP_000211838.1	antirestriction protein	-
JJW04_RS07020 (1473013)	1473013..1473252	-	240	WP_000194654.1	DUF905 family protein	-
JJW04_RS07025 (1473329)	1473329..1473796	-	468	WP_001547765.1	protein YkfB	-
JJW04_RS07030 (1473819)	1473819..1474262	-	444	WP_000824223.1	lipoprotein YafY	-
JJW04_RS07035 (1474262)	1474262..1474438	-	177	WP_001285112.1	hypothetical protein	-
JJW04_RS07040 (1474485)	1474485..1474676	-	192	Protein_1364	DeoR family transcriptional regulator	-
JJW04_RS07045 (1474893)	1474893..1475714	-	822	WP_000197389.1	DUF932 domain-containing protein	-
JJW04_RS07050 (1475806)	1475806..1476669	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T187044 WP_000854672.1 NZ_CP068281:c1471378-1471037 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T187044 NZ_CP089776:3766373-3766630 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT187044 WP_000070395.1 NZ_CP068281:c1471716-1471399 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT187044 NZ_CP089776:3766101-3766376 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684