Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4181427..4182081 Replicon chromosome
Accession NZ_CP068279
Organism Escherichia coli strain CY708

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JJW05_RS20310 Protein ID WP_000244777.1
Coordinates 4181427..4181834 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JJW05_RS20315 Protein ID WP_000354046.1
Coordinates 4181815..4182081 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJW05_RS20290 (4177384) 4177384..4179117 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
JJW05_RS20295 (4179123) 4179123..4179833 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JJW05_RS20300 (4179858) 4179858..4180754 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JJW05_RS20305 (4180866) 4180866..4181387 + 522 WP_001055874.1 flavodoxin FldB -
JJW05_RS20310 (4181427) 4181427..4181834 - 408 WP_000244777.1 protein YgfX Toxin
JJW05_RS20315 (4181815) 4181815..4182081 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JJW05_RS20320 (4182324) 4182324..4183304 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JJW05_RS20325 (4183500) 4183500..4184159 - 660 WP_000250274.1 hemolysin III family protein -
JJW05_RS20330 (4184323) 4184323..4184634 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JJW05_RS20335 (4184679) 4184679..4186112 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
JJW05_RS20340 (4186169) 4186169..4186912 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T187025 WP_000244777.1 NZ_CP068279:c4181834-4181427 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T187025 NZ_CP089776:2317302-2317712 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT187025 WP_000354046.1 NZ_CP068279:c4182081-4181815 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT187025 NZ_CP089776:2317076-2317309 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References