Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3235988..3236819 Replicon chromosome
Accession NZ_CP068279
Organism Escherichia coli strain CY708

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag JJW05_RS15875 Protein ID WP_000854814.1
Coordinates 3236445..3236819 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag JJW05_RS15870 Protein ID WP_001285584.1
Coordinates 3235988..3236356 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJW05_RS15855 (3233655) 3233655..3235187 + 1533 WP_001350525.1 protein YeeR -
JJW05_RS15860 (3235184) 3235184..3235630 + 447 WP_000187523.1 RadC family protein -
JJW05_RS15865 (3235693) 3235693..3235914 + 222 WP_000692323.1 DUF987 domain-containing protein -
JJW05_RS15870 (3235988) 3235988..3236356 + 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
JJW05_RS15875 (3236445) 3236445..3236819 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
JJW05_RS15880 (3236816) 3236816..3237010 + 195 WP_000988600.1 DUF5983 family protein -
JJW05_RS15885 (3237056) 3237056..3237136 + 81 Protein_3104 hypothetical protein -
JJW05_RS15890 (3237425) 3237425..3237553 - 129 Protein_3105 transposase domain-containing protein -
JJW05_RS15895 (3237673) 3237673..3237807 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
JJW05_RS15900 (3237908) 3237908..3238237 - 330 WP_000450409.1 DUF496 family protein -
JJW05_RS15905 (3238409) 3238409..3239467 - 1059 WP_001200891.1 FUSC family protein -
JJW05_RS15910 (3239665) 3239665..3240138 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
JJW05_RS15915 (3240257) 3240257..3241423 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T187020 WP_000854814.1 NZ_CP068279:3236445-3236819 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T187020 NZ_CP089776:c2195225-2194929 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT187020 WP_001285584.1 NZ_CP068279:3235988-3236356 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT187020 NZ_CP089776:c2195473-2195222 [Mycobacterium tuberculosis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References