Detailed information of TA system TA187009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2657530..2658168
• Replicon: chromosome
• Accession: NZ_CP068279
• Organism: Escherichia coli strain CY708

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JJW05_RS12920
• Protein ID: WP_000813794.1
• Coordinates: 2657530..2657706 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JJW05_RS12925
• Protein ID: WP_001270286.1
• Coordinates: 2657752..2658168 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJW05_RS12900 (2653149)	2653149..2654363	-	1215	WP_001326689.1	BenE family transporter YdcO	-
JJW05_RS12905 (2654416)	2654416..2654952	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
JJW05_RS12910 (2655025)	2655025..2656986	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JJW05_RS12915 (2657078)	2657078..2657308	-	231	WP_000494244.1	YncJ family protein	-
JJW05_RS12920 (2657530)	2657530..2657706	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JJW05_RS12925 (2657752)	2657752..2658168	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JJW05_RS12930 (2658247)	2658247..2659653	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
JJW05_RS12935 (2659898)	2659898..2661043	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
JJW05_RS12940 (2661061)	2661061..2662074	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
JJW05_RS12945 (2662075)	2662075..2663016	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T187009 WP_000813794.1 NZ_CP068279:2657530-2657706 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T187009 NZ_CP089776:1247533-1247907 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT187009 WP_001270286.1 NZ_CP068279:2657752-2658168 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT187009 NZ_CP089776:1247339-1247536 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• PDB: 6HPB