Detailed information of TA system TA186966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2939479..2940050
• Replicon: chromosome
• Accession: NZ_CP068249
• Organism: Enterococcus faecalis strain T90-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JG557_RS14445
• Protein ID: WP_002385138.1
• Coordinates: 2939479..2939820 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: JG557_RS14450
• Protein ID: WP_002354773.1
• Coordinates: 2939820..2940050 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG557_RS14440	2935494..2939108	-	3615	WP_010774058.1	DNA-directed RNA polymerase subunit beta	-
JG557_RS14445	2939479..2939820	-	342	WP_002385138.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JG557_RS14450	2939820..2940050	-	231	WP_002354773.1	hypothetical protein	Antitoxin
JG557_RS14455	2940456..2940671	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
JG557_RS14460	2940810..2941802	+	993	WP_002385136.1	biotin--[acetyl-CoA-carboxylase] ligase	-
JG557_RS14465	2942066..2942704	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
JG557_RS14470	2943392..2945008	+	1617	WP_002372620.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13128.35 Da
• Isoelectric Point: 9.3984

>T186966 WP_002385138.1 NZ_CP068249:c2939820-2939479 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAAICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T186966 NZ_CP089775:2329330-2329740 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT186966 WP_002354773.1 NZ_CP068249:c2940050-2939820 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT186966 NZ_CP089775:2329104-2329337 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812