Detailed information of TA system TA186964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 2605049..2606157
• Replicon: chromosome
• Accession: NZ_CP068249
• Organism: Enterococcus faecalis strain T90-1

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: JG557_RS12875
• Protein ID: WP_000233000.1
• Coordinates: 2605288..2606157 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: JG557_RS12870
• Protein ID: WP_000205227.1
• Coordinates: 2605049..2605273 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG557_RS12850	2600679..2602163	+	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-
JG557_RS12855	2602217..2603020	+	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
JG557_RS12860	2603329..2604611	-	1283	Protein_2505	ISL3 family transposase	-
JG557_RS12865	2604820..2604906	+	87	Protein_2506	site-specific recombinase	-
JG557_RS12870	2605049..2605273	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
JG557_RS12875	2605288..2606157	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
JG557_RS12880	2606138..2606872	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
JG557_RS12885	2606905..2607813	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
JG557_RS12890	2607810..2608352	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
JG557_RS12895	2608445..2609239	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
JG557_RS12900	2609515..2610087	+	573	WP_202074493.1	HTH domain-containing protein	-
JG557_RS12905	2610111..2610281	+	171	WP_000374053.1	hypothetical protein	-
JG557_RS12910	2610315..2610995	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	erm(B) / aac(6')-aph(2'') / ant(6)-Ia / lsa(E) / lnu(B) / aph(3')-III	-	2587968..2626396	38428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T186964 WP_000233000.1 NZ_CP068249:2605288-2606157 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T186964 NZ_CP089775:c2236566-2236222 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT186964 WP_000205227.1 NZ_CP068249:2605049-2605273 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT186964 NZ_CP089775:c2236808-2236560 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Structures

Toxin

• AlphaFold DB: R3JIN1