Detailed information of TA system TA186955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1513651..1514180
• Replicon: chromosome
• Accession: NZ_CP068247
• Organism: Staphylococcus sp. T93

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JG553_RS07455
• Protein ID: WP_000621175.1
• Coordinates: 1513651..1514013 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JG553_RS07460
• Protein ID: WP_000948331.1
• Coordinates: 1514010..1514180 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG553_RS07435	1510641..1511411	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
JG553_RS07440	1511386..1511865	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
JG553_RS07445	1511867..1512193	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JG553_RS07450	1512312..1513302	-	991	Protein_1432	PP2C family protein-serine/threonine phosphatase	-
JG553_RS07455	1513651..1514013	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JG553_RS07460	1514010..1514180	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JG553_RS07465	1514265..1515413	-	1149	WP_001281145.1	alanine racemase	-
JG553_RS07470	1515479..1515838	-	360	WP_000581200.1	holo-ACP synthase	-
JG553_RS07475	1515842..1516333	-	492	WP_001205910.1	PH domain-containing protein	-
JG553_RS07480	1516320..1517903	-	1584	WP_001294626.1	PH domain-containing protein	-
JG553_RS07485	1517896..1518375	-	480	WP_001287088.1	hypothetical protein	-
JG553_RS07490	1518584..1519144	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T186955 WP_000621175.1 NZ_CP068247:c1514013-1513651 [Staphylococcus sp. T93]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T186955 NZ_CP089775:1690966-1691283 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT186955 WP_000948331.1 NZ_CP068247:c1514180-1514010 [Staphylococcus sp. T93]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT186955 NZ_CP089775:1690667-1690969 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1