Detailed information of TA system TA186950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 80111..81219
• Replicon: plasmid pFac90-154
• Accession: NZ_CP068245
• Organism: Enterococcus faecium strain fac90

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: JG556_RS12935
• Protein ID: WP_000233000.1
• Coordinates: 80111..80980 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: JG556_RS12940
• Protein ID: WP_000205227.1
• Coordinates: 80995..81219 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG556_RS12905	75273..75953	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
JG556_RS12910	76181..76753	-	573	WP_202074493.1	HTH domain-containing protein	-
JG556_RS12915	77029..77823	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
JG556_RS12920	77916..78458	-	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
JG556_RS12925	78455..79363	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
JG556_RS12930	79396..80130	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
JG556_RS12935	80111..80980	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
JG556_RS12940	80995..81219	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
JG556_RS12945	81657..82939	+	1283	Protein_95	ISL3 family transposase	-
JG556_RS12950	83248..84051	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
JG556_RS12955	84105..85589	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T186950 WP_000233000.1 NZ_CP068245:c80980-80111 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T186950 NZ_CP089775:1246470-1246844 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT186950 WP_000205227.1 NZ_CP068245:c81219-80995 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT186950 NZ_CP089775:1246276-1246473 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: R3JIN1