Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 459604..460175 Replicon chromosome
Accession NZ_CP068244
Organism Enterococcus faecium strain fac90

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag JG556_RS02195 Protein ID WP_002302307.1
Coordinates 459834..460175 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag JG556_RS02190 Protein ID WP_002323011.1
Coordinates 459604..459834 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG556_RS02165 455093..456421 + 1329 WP_010718318.1 FAD-dependent oxidoreductase -
JG556_RS02170 456443..457069 + 627 WP_002286816.1 cysteine hydrolase -
JG556_RS02175 457252..457833 + 582 WP_002331663.1 TetR/AcrR family transcriptional regulator -
JG556_RS02180 458198..458773 + 576 WP_010718317.1 SOS response-associated peptidase family protein -
JG556_RS02185 458978..459316 - 339 WP_002286804.1 hypothetical protein -
JG556_RS02190 459604..459834 + 231 WP_002323011.1 hypothetical protein Antitoxin
JG556_RS02195 459834..460175 + 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JG556_RS02200 461025..461210 + 186 WP_002304207.1 hypothetical protein -
JG556_RS02210 462846..462971 + 126 WP_002352139.1 LPXTG cell wall anchor domain-containing protein -
JG556_RS02215 463045..463938 + 894 WP_002326335.1 class C sortase -
JG556_RS02220 464122..464796 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element ClpL ebpC 390867..574940 184073


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T186949 WP_002302307.1 NZ_CP068244:459834-460175 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T186949 NZ_CP089775:c1237831-1237520 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACACCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT186949 WP_002323011.1 NZ_CP068244:459604-459834 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT186949 NZ_CP089775:c1238151-1237831 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References