Detailed information of TA system TA186947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 924734..925263
• Replicon: chromosome
• Accession: NZ_CP068241
• Organism: Staphylococcus sp. 11-B-312

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A6N7XWP7
• Locus tag: JG554_RS04285
• Protein ID: WP_019468010.1
• Coordinates: 924901..925263 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A921LYV8
• Locus tag: JG554_RS04280
• Protein ID: WP_046207786.1
• Coordinates: 924734..924904 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG554_RS04255	920545..921024	+	480	WP_070039503.1	PH domain-containing protein	-
JG554_RS04260	921017..922522	+	1506	WP_070039501.1	PH domain-containing protein	-
JG554_RS04265	922515..923006	+	492	WP_046207783.1	PH domain-containing protein	-
JG554_RS04270	923041..923391	+	351	WP_046207784.1	holo-ACP synthase	-
JG554_RS04275	923498..924646	+	1149	WP_070039499.1	alanine racemase	-
JG554_RS04280	924734..924904	+	171	WP_046207786.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JG554_RS04285	924901..925263	+	363	WP_019468010.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JG554_RS04290	925606..926607	+	1002	WP_046207787.1	PP2C family protein-serine/threonine phosphatase	-
JG554_RS04295	926676..927002	+	327	WP_046207788.1	anti-sigma factor antagonist	-
JG554_RS04300	927004..927486	+	483	WP_064209857.1	anti-sigma B factor RsbW	-
JG554_RS04305	927461..928231	+	771	WP_046207790.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13595.69 Da
• Isoelectric Point: 9.9426

>T186947 WP_019468010.1 NZ_CP068241:924901-925263 [Staphylococcus sp. 11-B-312]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSEEKMQEVNNAIDISLGLHNIRNHRT

Nucleotide

• Length: 363 bp

>T186947 NZ_CP089775:1020364-1020798 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6332.00 Da
• Isoelectric Point: 4.0121

>AT186947 WP_046207786.1 NZ_CP068241:924734-924904 [Staphylococcus sp. 11-B-312]
MASFNQSRNHSIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYIISKNNNE

Nucleotide

• Length: 171 bp

>AT186947 NZ_CP089775:1020179-1020358 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Toxin

• AlphaFold DB: A0A6N7XWP7