Detailed information of TA system TA186946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 913356..913870
• Replicon: chromosome
• Accession: NZ_CP068241
• Organism: Staphylococcus sp. 11-B-312

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: A0A921J8Q4
• Locus tag: JG554_RS04225
• Protein ID: WP_046207776.1
• Coordinates: 913664..913870 (+)
• Length: 69 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A921J6E7
• Locus tag: JG554_RS04220
• Protein ID: WP_046207775.1
• Coordinates: 913356..913649 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG554_RS04200	909017..909655	+	639	WP_070039511.1	thiamine phosphate synthase	-
JG554_RS04205	909742..910611	+	870	WP_046207772.1	membrane protein insertase YidC	-
JG554_RS04210	910809..911453	-	645	WP_070039509.1	HD domain-containing protein	-
JG554_RS04215	911731..913215	-	1485	WP_046207774.1	cardiolipin synthase	-
JG554_RS04220	913356..913649	+	294	WP_046207775.1	copper-sensing transcriptional repressor CsoR	Antitoxin
JG554_RS04225	913664..913870	+	207	WP_046207776.1	heavy-metal-associated domain-containing protein	Toxin
JG554_RS04230	914128..914268	+	141	WP_099091318.1	hypothetical protein	-
JG554_RS04235	914535..915722	-	1188	WP_203265377.1	rod shape-determining protein RodA	-
JG554_RS04240	915982..917052	+	1071	WP_046207778.1	D-alanine--D-alanine ligase	-
JG554_RS04245	917065..918420	+	1356	WP_070039505.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (20-65)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7973.09 Da
• Isoelectric Point: 4.3611

>T186946 WP_046207776.1 NZ_CP068241:913664-913870 [Staphylococcus sp. 11-B-312]
MESQTIYLRKIDNKQDKLQIERTLSQIIGVNHVSVEIEKAQVFIEFETPASLNNLEKDLYDLGFEILY

Nucleotide

• Length: 207 bp

>T186946 NZ_CP089775:846618-847046 [Mycobacterium tuberculosis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11214.89 Da
• Isoelectric Point: 6.8617

>AT186946 WP_046207775.1 NZ_CP068241:913356-913649 [Staphylococcus sp. 11-B-312]
MAEMQKAHHSEQIKSNLKSRLNRIEGQVRAINRMIDEDVYCDDVLTQIRATRSALNSVATKLLDHHMKSCIMDKVNQGEQ
QEAMDELLVTFQKLMKD

Nucleotide

• Length: 294 bp

>AT186946 NZ_CP089775:846337-846594 [Mycobacterium tuberculosis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA