Detailed information of TA system TA186942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1487686..1488272
• Replicon: chromosome
• Accession: NZ_CP068239
• Organism: Pseudomonas aeruginosa strain PA19-3047

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: JJQ57_RS06900
• Protein ID: WP_003120987.1
• Coordinates: 1487686..1487985 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: JJQ57_RS06905
• Protein ID: WP_003448662.1
• Coordinates: 1487982..1488272 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJQ57_RS06880	1482802..1483335	-	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-
JJQ57_RS06885	1483325..1484650	-	1326	WP_003099758.1	type 4b pilus protein PilO2	-
JJQ57_RS06890	1484654..1486363	-	1710	WP_010792227.1	PilN family type IVB pilus formation outer membrane protein	-
JJQ57_RS06895	1486363..1487484	-	1122	WP_003448658.1	TcpQ domain-containing protein	-
JJQ57_RS06900	1487686..1487985	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JJQ57_RS06905	1487982..1488272	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
JJQ57_RS06910	1488343..1488687	-	345	WP_003448665.1	hypothetical protein	-
JJQ57_RS06915	1488828..1490804	-	1977	WP_042931628.1	DEAD/DEAH box helicase	-
JJQ57_RS06920	1490801..1492690	-	1890	WP_042931627.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1432681..1516934	84253

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T186942 WP_003120987.1 NZ_CP068239:1487686-1487985 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T186942 NZ_CP089775:c758829-758446 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT186942 WP_003448662.1 NZ_CP068239:1487982-1488272 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT186942 NZ_CP089775:c759079-758924 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Toxin

• AlphaFold DB: G8CP73